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MABC1609-25UG Anti-AIF Antibody, clone 3C11

Detect AIF using this mouse monoclonal Anti-AIF, clone 3C11 Antibody, Cat. No. MABC1609-25UG, validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Anti-AIF Antibody, clone 3C11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: AIFM1, AIF, PDCD8 UniProt Number: O95831
MABC1609-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1609-25UG
DescriptionAnti-AIF Antibody, clone 3C11
Alternate Names
  • Apoptosis-inducing factor 1
  • mitochondrial
  • Programmed cell death protein 8
Background InformationApoptosis-inducing factor 1, mitochondrial (UniProt: O95831; also known as Programmed cell death protein 8) is encoded by the AIFM1 (also known as AIF, PDCD8) gene (Gene ID: 9131) in human. AIF is a nuclear encoded flavoprotein that is generally confined to the mitochondrial intermembrane space and functions both as NADH oxidoreductase and as regulator of apoptosis. Six isoformsi of AIF have been described that are produced by alternative splicing. Under normal conditions, a 54-residue N-terminal (transit peptide) segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase to form the inner-membrane-anchored mature form (AIFmit). Upon induction of apoptosis, it is further proteolytically processed at to generate the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner. AIF also functions as an anti-apoptotic factor in normal mitochondria via its NADH oxidoreductase activity. Once released from mitochondria it translocates to the nucleus to induce caspase-independent fragmentation of chromosomal DNA. Mutations in AIF gene are linked to combined oxidative phosphorylation deficiency 6 that is characterized by psychomotor delay, hypotonia, areflexia, and muscle weakness and wasting. Defects in this gene are also known to cause Cowchock syndrome that leads to early childhood onset of a slowly progressive axonal sensorimotor neuropathy.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect AIF using this mouse monoclonal Anti-AIF, clone 3C11 Antibody, Cat. No. MABC1609-25UG, validated for use in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected AIF in human skin, human testis, and human kidney tissue.
Biological Information
ImmunogenHis-tagged full length recombinant human AIF.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3C11 specificallly detects AIF in human cells.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • AIFM1
  • AIF
  • PDCD8
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected AIF in 10 µg of A431 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1609-25UG 04054839437878