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ST1049 Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ST1049
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, Rb, R, YeastMMonoclonal Antibody
Description
OverviewRecognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.
Catalogue NumberST1049
Brand Family Calbiochem®
Application Data
Detection of human 20S Proteasome α1, 2, 3, 5, 6, 7-subunits by 2D PAGE and immunoblotting. Sample: Whole tissue extract from human placental tissue. Primary antibody: Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) (Cat. No. ST1049) (1:1000). Detection: luminography.
References
ReferencesKopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHuman placental tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunohistochemistry/Immunofluorescence Kabashi, E., et al. 2004. J. Neurochem. 89, 1325. Frozen Sections Fratta, P., et al. 2005. Am. J. Pathol. 167, 517. Immunocytochemistry/Immunofluorescence Taylor, D.M., et al. 2005. Cell Stress Chaperones 10, 230.
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Not Immunoprecipitation
Application NotesImmunoblotting (1:1000, chemiluminescence)
Immunoprecipitation (not recommended)
ELISA (see comments)
Immunofluorescence (see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Frozen Sections (see application references)
Application CommentsImmunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogendinitrophenylated proteasomes
ImmunogenHuman
Epitopewithin the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
CloneMCP231
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rabbit
  • Rat
  • Yeast
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1049 0

Documentation

Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) SDS

Title

Safety Data Sheet (SDS) 

Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) Certificates of Analysis

TitleLot Number
ST1049

References

Reference overview
Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.

Brochure

Title
Proteasome/Ubiquitination NF-kB Pathway Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-November-2008 JSW
ApplicationImmunoblotting (1:1000, chemiluminescence)
Immunoprecipitation (not recommended)
ELISA (see comments)
Immunofluorescence (see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Frozen Sections (see application references)
Application Data
Detection of human 20S Proteasome α1, 2, 3, 5, 6, 7-subunits by 2D PAGE and immunoblotting. Sample: Whole tissue extract from human placental tissue. Primary antibody: Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) (Cat. No. ST1049) (1:1000). Detection: luminography.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome.
BackgroundProteasomes are large multi-subunit complexes, that are localized in the nucleus and cytosol, and selectively degrade intracellular proteins. They play a major role in the degradation of many proteins involved in cellular processes such as cell cycling, apoptosis, and proliferation. The human proteasome contains two copies of each of seven different α-type and β-type subunits assembled to form a stack of four seven-membered rings. The α-subunits comprise the outer rings while the β-subunits comprise the inner rings. The α-subunits contain information for the binding of regulatory complexes such as PA700 and PA28.
HostMouse
Immunogen speciesHuman
Immunogendinitrophenylated proteasomes
Epitopewithin the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
CloneMCP231
IsotypeIgG₁
Specieshuman, mouse, rabbit, rat, yeast
Positive controlHuman placental tissue
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
CommentsImmunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Highly Toxic
ReferencesKopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
Application referencesImmunohistochemistry/Immunofluorescence Kabashi, E., et al. 2004. J. Neurochem. 89, 1325. Frozen Sections Fratta, P., et al. 2005. Am. J. Pathol. 167, 517. Immunocytochemistry/Immunofluorescence Taylor, D.M., et al. 2005. Cell Stress Chaperones 10, 230.