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129935 Actinomycin D, 7-Amino- - CAS 7240-37-1 - Calbiochem

Actinomycin D, 7-Amino, CAS 7240-37-1, is a DNA intercalator that complexes with DNA. Absorbs at 555 nm & emits at 655 nm. Exhibits growth-inhibitory activity against certain leukemias and sarcomas.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C₁, 7-Aminodactinomycin

Primary Target: leukemias and sarcomas
129935
  
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
7240-37-1C₆₂H₈₇N₁₃O₁₆
Description
Overview7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. Fluorescent excitation and emission maxima are 500 and 672 nm, respectively. Complexes with DNA and absorbs at 555 nm and emits at 655 nm. Useful in distinguishing early apoptotic cells, which have lost membrane integrity, from apoptotic and live cells. Has been used in DNA analysis and as a probe for chromosome structure and function.
Catalogue Number129935
Brand Family Calbiochem®
Synonyms7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C₁, 7-Aminodactinomycin
References
ReferencesSchmid, I., et al. 1994. J. Immunol. Methods 170, 145.
Berg, R.J., et al. 1993. Carcinogenesis 14, 103.
Chen, F.M. 1992. Biochemistry 31, 6223.
Schmid, I., et al. 1992. Cytometry 13, 204.
Toba, K., et al. 1992. Cytometry 13, 60.
Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
Product Information
CAS number7240-37-1
FormReddish-purple solid
Hill FormulaC₆₂H₈₇N₁₃O₁₆
Chemical formulaC₆₂H₈₇N₁₃O₁₆
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationActinomycin D, 7-Amino, CAS 7240-37-1, is a DNA intercalator that complexes with DNA. Absorbs at 555 nm & emits at 655 nm. Exhibits growth-inhibitory activity against certain leukemias and sarcomas.
Biological Information
Primary Targetleukemias and sarcomas
Purity≥85% by HPLC
Sample TypeIntact Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSAU1579000
Safety Information
R PhraseR: 28

Very toxic if swallowed.
S PhraseS: 36/37-45-28

Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot, purge with inert gas and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
129935 0

Documentation

Actinomycin D, 7-Amino- - CAS 7240-37-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Actinomycin D, 7-Amino- - CAS 7240-37-1 - Calbiochem Certificates of Analysis

TitleLot Number
129935

References

Reference overview
Schmid, I., et al. 1994. J. Immunol. Methods 170, 145.
Berg, R.J., et al. 1993. Carcinogenesis 14, 103.
Chen, F.M. 1992. Biochemistry 31, 6223.
Schmid, I., et al. 1992. Cytometry 13, 204.
Toba, K., et al. 1992. Cytometry 13, 60.
Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Jeffrey D. Price, et al. (2005) Induction of a regulatory phenotype in human CD4+ T cells by Streptococcal M protein. Journal of immunology 175, 677-684.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision26-August-2010 JSW
    Synonyms7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C₁, 7-Aminodactinomycin
    Description7-Aminoactinomycin D is a membrane impermeable fluorescent DNA intercalator. Like its parent compound, Actinomycin D (Cat. No. 114666), 7-AAD has antibacterial properties and growth inhibitory activity against certain leukemias and sarcomas. Useful for flow cytometry and fluorescence microscopy. Used for staining nonviable cells and for identifying apoptotic cells. Compatible with FITC and PE cell surface staining. The absorption/emission maxima are as follows: unbound (ex. 500 nm, em. 672 nm) and DNA bound (ex. 555 nm, em. 655 nm). λmax 528 nm ± 2; εmax = 22,700 ± 5,000 M-1 cm-1
    FormReddish-purple solid
    CAS number7240-37-1
    RTECSAU1579000
    Chemical formulaC₆₂H₈₇N₁₃O₁₆
    Structure formulaStructure formula
    Purity≥85% by HPLC
    SolubilityDMSO (20 mg/ml) or Ethanol (5 mg/ml). Further dilute in aqueous buffers just prior to use.
    Storage Protect from light
    -20°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution aliquot, purge with inert gas and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
    Toxicity Highly Toxic
    Merck USA index14, 2800
    ReferencesSchmid, I., et al. 1994. J. Immunol. Methods 170, 145.
    Berg, R.J., et al. 1993. Carcinogenesis 14, 103.
    Chen, F.M. 1992. Biochemistry 31, 6223.
    Schmid, I., et al. 1992. Cytometry 13, 204.
    Toba, K., et al. 1992. Cytometry 13, 60.
    Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
    Citation
  • Jeffrey D. Price, et al. (2005) Induction of a regulatory phenotype in human CD4+ T cells by Streptococcal M protein. Journal of immunology 175, 677-684.
    		•