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171536 AMPK (α1/β1/γ1), His•Tag®, Human, Recombinant, S. frugiperda

AMPK (α1/β1/γ1), His•Tag®, Human, Recombinant, is a full length enzyme. Consists of subunits A1, B1, & G1, each fused at the C-terminus to a His•Tag® sequence & co-expressed in S. frugiperda.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
171536
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Overview

Products

Catalogue NumberPackaging Qty/Pack
171536-5UG Plastic ampoule 5 μg
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Description
OverviewFull length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag® sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1)
Catalogue Number171536
Brand Family Calbiochem®
Application Data

The specific activity of AMPK was measured as outlined in the Recommended Reaction Conditions.
References
ReferencesScott J.W., 2007. EMBO J 26, 806.
Minokoshi Y et al. 2004. Nature 428, 569.
Product Information
FormLiquid
FormulationIn 300 mM NaCl, 150 mM Imidazole, 50 mM Sodium Phosphate, 0.2 mM DTT, 0.1 mM PMSF, 25% glycerol, pH 7.0.
Quality LevelMQ100
Biological Information
Purity≥90% by SDS-PAGE
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
171536-5UG 04055977207248