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AG533 ADAM 10, control peptide for AB19026

ADAM 10, control peptide for AB19026 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Gene Symbol: ADAM10, CDw156, CD156c, HsT18717, kuzbanian, kuz, MADM, KUZ, EC 3.4.24.81 UniProt Number: O14672
AG533
50 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberAG533
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionADAM 10, control peptide for AB19026
OverviewControl peptide for Chemicon Cat. No. AB19026 (Rabbit anti-ADAM10). Peptide corresponds to amino acids 732-748 near the C-terminus.
References
Product Information
PresentationLiquid in PBS, pH 7.2, containing 0.1% BSA and 0.02% sodium azide.
Quality LevelMQ100
Applications
Key Applications
  • Peptide Inhibition Assay
Application NotesBlocking: Use 1 μg control peptide per 1 μg antibody . Control peptide, because of it's small size (2-3 kDa), is not recommended for Western.

Optimal working dilutions must be determined by end user.
Biological Information
Concentration0.2 mg/mL
SourceSynthetic peptide
Entrez Gene Number
Entrez Gene SummaryMembers of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin.
Gene Symbol
  • ADAM10
  • CDw156
  • CD156c
  • HsT18717
  • kuzbanian
  • kuz
  • MADM
  • KUZ
  • EC 3.4.24.81
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14672 # Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala- -Val-77' to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity).
COFACTOR: Binds 1 zinc ion (By similarity).
SIZE: 748 amino acids; 84142 Da
SUBUNIT: Interacts with ephrin-A2 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Intracytoplasmic membrane; Single-pass type I membrane protein. Note=Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
TISSUE SPECIFICITY: Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
DOMAIN: SwissProt: O14672 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Contains 1 disintegrin domain. & Contains 1 peptidase M12B domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AG533 04053252469329

Documentation

ADAM 10, control peptide for AB19026 SDS

Title

Safety Data Sheet (SDS) 

ADAM 10, control peptide for AB19026 Certificates of Analysis

TitleLot Number
HUMAN ADAM10 CONTROL PEPTIDE FOR AB19026 - 2376017 2376017
HUMAN ADAM10 CONTROL PEPTIDE FOR AB19026 2969350
HUMAN ADAM10 CONTROL PEPTIDE FOR AB19026 - 2186339 2186339
HUMAN ADAM10 CONTROL PEPTIDE FOR AB19026 - 2224431 2224431
HUMAN ADAM10 CONTROL PEPTIDE FOR AB19026 - 2272129 2272129
HUMAN ADAM10 CONTROL PEPTIDE FOR AB19026 - 2283862 2283862
HUMAN ADAM10 CONTROL PEPTIDE FOR AB19026 - 2352400 2352400
HUMAN ADAM10 CONTROL PEPTIDE FOR AB19026 - 3829502 3829502

References

Reference overviewPub Med ID
Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteinases
Rosendahl, M.S.
J. Biol. Chem., 272:24588-24593 (1997)  1997

9305925 9305925