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70609 pETBlue™-2 DNA (uncut) - Novagen

Novagen's pETBlue™-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes.

pETBlue™-2 DNA (uncut) - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70609
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
70609-3 Ampulka plastikowa 20 μg
Description
OverviewThe pETBlue™-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV•Tag® and His•Tag® sequences. The sequence is numbered from the first base of the T7 promoter sequence.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number70609
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
70609-3 04055977257465

Documentation

pETBlue™-2 DNA (uncut) - Novagen MSDS

Title

Safety Data Sheet (SDS) 

pETBlue™-2 DNA (uncut) - Novagen Certificates of Analysis

TitleLot Number
70609

Citations

Tytuł
  • Yoshitaka Sato, et al. (2006) Sumoylation of CCAAT/enhancer-binding protein alpha and its functional roles in hepatocyte differentiation. Journal of Biological Chemistry 281, 21629-21639.
  • Michael D. Urbaniak, et al. (2005) The N-Acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis Is a zinc metalloenzyme. Journal of Biological Chemistry 280, 22831-22838.
  • Cynthia L. Richard, Animesh Tandon and Robert G. Kranz. (2004) Rhodobacter capsulatus nifA1 promoter: high-GC -10 regions in High-GC bacteria and the basis for their transcription. Journal of Bacteriology 186, 740-749.
  • Céline Pujol and James B. Bliska. (2003) The ability to replicate in macrophages Is conserved between Yersinia pestis and Yersinia pseudotuberculosis. Infection and Immunity 71, 5892-5899.
  • Xianghong Li and A-Lien Lu. (2001) Molecular cloning and functional analysis of the mutY homolog of Deinococcus radiodurans. Journal of Bacteriology 183, 6151-6158.
  • Sheng Yuan, Yajun Wu and Daniel J. Cosgrove. (2001) A fungal endoglucanase with plant cell wallextension activity. Plant Physiology 127, 324-333.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB249 pETBlue™ System Manual

    Vector Map

    Title
    TB259VM pETBlue™-2 Vector Map