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14-770M mTOR (1362-end) Protein, active, 100 µg

Active, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.

mTOR (1362-end) Protein, active, 100 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-770M
100 µg  Also available in 10 μg size (cat#14-770) and in bulk (cat# 14-770-K).
Purchase on Sigma-Aldrich

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Replacement Information
Description
Catalogue Number14-770M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionmTOR (1362-end) Protein, active, 100 µg
OverviewN-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end
Alternate Names
  • FRAP1
References
Product Information
Presentation16µg of enzyme in 100µl of 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300µg/ml FLAGTM peptide, 20% v/v glycerol
Quality LevelMQ100
Applications
ApplicationActive, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Specific Activity377Units/mg, where one unit of mTOR activity is defined as 1 nmole of phosphate incorporated into 2mg/ml mTOR substrate per minute at 30°C with a final ATP concentration of 100µM.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
Gene Symbol
  • FRAP1
  • RAPT1
  • MTOR
  • FRAP
  • mTOR
  • FLJ44809
  • FRAP2
  • RAFT1
Protein TargetmTOR (FRAP1)
Target Sub-FamilyLipid/Atypical
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
SIZE: 2549 amino acids; 288892 Da
SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Packaging Information
Material Size100 µg
Material PackageAlso available in 10 μg size (cat#14-770) and in bulk (cat# 14-770-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
14-770M 04053252337680

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Numer katalogowy Opis
14-770 mTOR (1362-end) Protein, active, 10 µg

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Kategorie

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases