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23-025 UbcH4 Protein, active, 50 µg

Active, full length, recombinant human UbcH4. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

UbcH4 Protein, active, 50 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
23-025
50 µg  
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Key ApplicationsSpeciesCascade Component
Enzyme AssaysHuman E2
Description
Catalogue Number23-025
Brand Family Upstate
Trade Name
  • Upstate
DescriptionUbcH4 Protein, active, 50 µg
OverviewC-terminal cMyc and 6His-tagged, recombinant human Smac/DIABLO amino acids 56-end, expressed in E.coli
Alternate Names
  • Ubiquitin-conjugating enzyme E2 D2
  • Ubiquitin-protein ligase D2
  • UbcH5b
  • Ubiquitin-conjugating enzyme E2-17kDa 2
  • UBE2D2
Background InformationUbiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. The UbcH5 family (UbcH5a, UbcH5b/UbcH4 and UbcH5c) have been shown to be the most active class of E2 enzymes in cell extracts and are associated with the regulation of a number of proteins associated with cell signalling, including p53, IκBα and β-catenin.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, full length, recombinant human UbcH4. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications
  • Enzyme Assays
Biological Information
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade ComponentE2
Entrez Gene Number
Purification MethodPurified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
23-025 04053252294709

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Alternative Packsize

Numer katalogowy Opis
23-025M UbcH4 Protein, active, 250 µg

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Ubiquitin Proteasome System: Enzymes and Substrates

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Kategorie

Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitin E2 Conjugating Enzymes
Life Science Research > Proteins and Enzymes > Ubiquitin E2 Conjugating Enzymes