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23-021M UBE1 Protein, active, 250 µg

Active, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays.

UBE1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
23-021M
250 µg  
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Key ApplicationsSpeciesCascade Component
Enzyme AssaysHuman E1
Description
Catalogue Number23-021M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionUBE1 Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant human UBE1 full length, expressed by baculovirus in Sf21 insect cells.
Alternate Names
  • Ubiquitin activating enzyme E1
  • Ubiquitin-like modifier-activating enzyme 1
  • UBA1
  • A1S9 Protein
Background InformationUBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It is responsible for the primary ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. UBE1 activates ubiquitin by the ATP dependent adenylation of its C-terminal glycine carboxyl group. This ubiquitin residue is then linked to the sulphydryl side chain moiety of a cysteine residue within UBE1 by forming a high energy thiol ester bond, liberating free AMP. There are two active sites within the UBE1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays.
Key Applications
  • Enzyme Assays
Biological Information
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade ComponentE1
Entrez Gene Number
Purification MethodPurified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size250 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
23-021M 04053252632945

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Kategorie

Life Science Research > Proteins and Enzymes > Ubiquitin E1 Activating Enzyme
Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitin E1 Activating Enzyme