Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

616376 TRAIL, Human, Recombinant, NSO cells

TRAIL, Human, Recombinant, NSO cells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Apo2L; TNF-Related Apoptosis-Inducing Ligand

616376
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
616376-10UG Ampulka plastikowa 10 μg
Description
OverviewRecombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.
Catalogue Number616376
Brand Family Calbiochem®
SynonymsApo2L; TNF-Related Apoptosis-Inducing Ligand
References
ReferencesGriffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
Pan, G., et al. 1997. Science 277, 815.
Pan, G., et al. 1997. Science 276, 111.
Sheridan, J.P., et al. 1997. Science 277, 818.
Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1 EU/µg TRAIL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
616376-10UG 04055977186253

Documentation

TRAIL, Human, Recombinant, NSO cells MSDS

Title

Safety Data Sheet (SDS) 

TRAIL, Human, Recombinant, NSO cells Certificates of Analysis

TitleLot Number
616376

References

Przegląd literatury
Griffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
Pan, G., et al. 1997. Science 277, 815.
Pan, G., et al. 1997. Science 276, 111.
Sheridan, J.P., et al. 1997. Science 277, 818.
Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Tytuł
  • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
  • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision12-September-2008 RFH
    SynonymsApo2L; TNF-Related Apoptosis-Inducing Ligand
    DescriptionRecombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.
    FormLyophilized
    FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
    Purity≥97% by SDS-PAGE
    ContaminantsEndotoxin: ≤1 EU/µg TRAIL
    Biological activityED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
    SolubilityReconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.
    Storage ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
    Toxicity Standard Handling
    ReferencesGriffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
    Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
    Pan, G., et al. 1997. Science 277, 815.
    Pan, G., et al. 1997. Science 276, 111.
    Sheridan, J.P., et al. 1997. Science 277, 818.
    Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
    Citation
  • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
  • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.
    		•