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613548 TMB, Insoluble

TMB, Insoluble MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3,3ʹ,5,5ʹ-Tetramethylbenzidine

613548
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Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
613548-100ML Butelka szklana 100 ml
Description
OverviewPeroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.
Catalogue Number613548
Brand Family Calbiochem®
Synonyms3,3ʹ,5,5ʹ-Tetramethylbenzidine
References
Product Information
CAS number54827-17-7
FormClear to light brown liquid
Formulation1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSDV2300000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Carcinogenic / Teratogenic
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsDiscard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
613548-100ML 04055977263800

Documentation

TMB, Insoluble MSDS

Title

Safety Data Sheet (SDS) 

TMB, Insoluble Certificates of Analysis

TitleLot Number
613548
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-September-2008 RFH
Synonyms3,3ʹ,5,5ʹ-Tetramethylbenzidine
DescriptionInsoluble TMB substrate supplied as a single component system. TMB is the most sensitive chromogenic substrate for detection of horseradish peroxidase (HRP)-labeled probes. In the presence of HRP and hydrogen peroxide (H2O2), TMB is oxidized first to a blue, cation free radical; upon further reaction with HRP/H2O2, or addition of acid, the radical is converted to a yellow diimine terminal oxidation product. TMB is used routinely in ELISA procedures, whereby its soluble oxidation products are measured spectrophotometrically. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate or other sugar polymers are usually added.This TMB reagent is a safe, single component system that has been developed for use in blotting procedures by a proprietary methodology that eliminates the use of sugar polymers. The reagent produces a stable, insoluble aquamarine precipitate at the reaction site with little or no background. It can significantly increase the detection limits of assays on a variety of membranes. Ideal for many immunoblotting procedures and can significantly increase detection limits of assays on a variety of membranes
FormClear to light brown liquid
Formulation1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.
Recommended reaction conditionsSuggested Procedure for Immunoblot Staining with TMB: 1. Complete all required incubations with antibodies and HRP-labeled probes. 2. Wash membranes thoroughly with phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), containing 0.1% Tween®-20 detergent. Note: Do not use reagents containing thimerosal or phenolic compounds as preservatives. 3. Following the final wash, cover the membranes completely with TMB solution and incubate with gentle rocking for 5-30 min, or until the desired color intensity is obtained (aquamarine/blue bands or dots will appear at the sites of enzyme activity). Note: Variables associated with assay conditions will dictate the proper reaction time. If the color develops immediately, the TMB precipitate may flake off the membrane. If this occurs, further dilution of the HRP probe is recommended. A fine blue line circumscribing the band or dot also suggests that dilution of the enzyme is necessary. 4. Stop the reaction by washing the membrane thoroughly in distilled water. DO NOT WASH WITH BUFFERED SOLUTIONS OR TAP WATER. These will cause fading. Also, DO NOT USE ACIDS TO STOP THE REACTION. Acids will turn the bands yellow. Excessive background staining indicates incomplete removal of unbound HRP from membranes. To remedy this problem, increase the wash steps or washing times. 5. Air-dry membranes and store protected from light.
CAS number54827-17-7
RTECSDV2300000
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsDiscard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.
Toxicity Carcinogenic / Teratogenic