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PF019 TIMP-1, Human, Recombinant, CHO Cells

Recombinant, human TIMP-1 expressed in CHO cells.

TIMP-1, Human, Recombinant, CHO Cells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Tissue Inhibitor of Metalloproteinase-1

Primary Target: Human MMP 1
PF019
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
PF019-3UG Ampulka plastikowa 3 μg
Description
OverviewRecombinant, human TIMP-1 expressed in CHO cells. Migrates as a 28 kDa protein under reducing conditions on SDS-PAGE.
Catalogue NumberPF019
Brand Family Calbiochem®
SynonymsTissue Inhibitor of Metalloproteinase-1
References
ReferencesFreudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250.
Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341.
Docherty, A.J.P. et al. 1985. Nature 318, 66.
Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.
Product Information
ATP CompetitiveN
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLyophilized
FormulationLyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 µg BSA, pH 7.3.
PreservativeNone
ReversibleN
Quality LevelMQ100
Applications
Application ReferencesInhibition Studies Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.
Application NotesImmunoblotting Standard (20 µl/lane)
MMP Inhibition (assay dependent, see comments)
Application CommentsTIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC50 of 1-5 nM (see application references).
Biological Information
Biological activityIC₅₀ against native human MMP-1 is ~1-5 nM.
Primary TargetHuman MMP 1
Primary Target IC<sub>50</sub>1-5 nM against human MMP 1
Purity≥95% by SDS PAGE (prior to lyophilization)
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
PF019-3UG 04055977207934

Documentation

TIMP-1, Human, Recombinant, CHO Cells MSDS

Title

Safety Data Sheet (SDS) 

TIMP-1, Human, Recombinant, CHO Cells Certificates of Analysis

TitleLot Number
PF019

References

Przegląd literatury
Freudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250.
Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341.
Docherty, A.J.P. et al. 1985. Nature 318, 66.
Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.

Citations

Tytuł
  • William J. Lane, et al. (2000) Stromal-derived factor 1-induced megakaryocyte migration and platelet production is dependent on matrix metalloproteinases. Blood 96, 4152-4159.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision06-September-2007 JSW
    SynonymsTissue Inhibitor of Metalloproteinase-1
    ApplicationImmunoblotting Standard (20 µl/lane)
    MMP Inhibition (assay dependent, see comments)
    DescriptionRecombinant, human TIMP-1 expressed in CHO cells. Migrates as a ~28 kDa protein by SDS-PAGE. The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a ~28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
    BackgroundThe activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
    FormLyophilized
    FormulationLyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 µg BSA, pH 7.3.
    Recommended reaction conditions
    Immunoblotting Standard Useful as an immunoblotting standard; use at 20 µl/lane for most applications. MMP Inhibition Assays TIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC50 of 1-5 nM (see application references).
    Purity≥95% by SDS PAGE (prior to lyophilization)
    Biological activityIC₅₀ against native human MMP-1 is ~1-5 nM.
    SolubilityReconstitute with PBS to the desired concentration and mix gently until completely dissolved.
    PreservativeNone
    CommentsTIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC50 of 1-5 nM (see application references).
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
    Toxicity Standard Handling
    ReferencesFreudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250.
    Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
    Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341.
    Docherty, A.J.P. et al. 1985. Nature 318, 66.
    Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.
    Citation
  • William J. Lane, et al. (2000) Stromal-derived factor 1-induced megakaryocyte migration and platelet production is dependent on matrix metalloproteinases. Blood 96, 4152-4159.
    		•
    Application referencesInhibition Studies Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.