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596105 THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound

THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: THIOLYTE® MB Reagent, MBB

596105
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Przegląd

Replacement Information

Tabela kluczowych gatunków

CAS #Purity
71418-44-5≥95% by HPLC

Products

Numer katalogowyOpakowanie Ilość/opak.
596105-100MG Ampulka plastikowa 100 mg
596105-250MG Ampulka plastikowa 250 mg
596105-25MG Ampulka plastikowa 25 mg
Description
OverviewHas been used to label E. coli thioredoxin. Weakly fluorescent but reacts with thiols to yield highly fluorescent stable thioethers.
Catalogue Number596105
Brand Family Calbiochem®
SynonymsTHIOLYTE® MB Reagent, MBB
References
ReferencesMansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
Chinn, P.C. 1985. Fed. Proc. 44, 1620.
Product Information
CAS number71418-44-5
FormYellow solid
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
596105-100MG 07790788051884
596105-250MG 07790788051891
596105-25MG 07790788051907

Documentation

THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound MSDS

Title

Safety Data Sheet (SDS) 

THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound Certificates of Analysis

TitleLot Number
596105

References

Przegląd literatury
Mansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
Chinn, P.C. 1985. Fed. Proc. 44, 1620.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-April-2008 RFH
SynonymsTHIOLYTE® MB Reagent, MBB
DescriptionContains a reactive functional group (allylic bromide) that is capable of reacting with a wide variety of nucleophiles to form stable covalent adducts. Reacts extremely rapidly with thiol groups under physiological conditions to yield highly fluorescent products. A labeling reaction is typically carried out at room temperature for 2 to 60 min, pH 7.4, using an equivalent amount (or a small excess) of reagent relative to the amount of thiol. Material is chemically and photolytically stable and shows an absorption maxima at 370 to 385 nm and emits light at 477 to 484 nm (turquoise). Readily penetrates membranes. Useful as an alternative tool in labeling methodology, complementing the use of such well known fluorescent labeling reagents as fluorescein and rhodamine isothiocyanates (whose reactions are slower and yield products that fade relatively fast during irradiation) and fluorescamine (which reacts primarily with amine groups, but whose products also fade relatively rapidly).
FormYellow solid
CAS number71418-44-5
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityAcetonitrile (13 mg/ml) or Methanol (25 mg/ml). Recommended stock solution is 13 mg/ml (50 mM) in acetonitrile.
Storage Protect from light
+15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
Toxicity Standard Handling
ReferencesMansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
Chinn, P.C. 1985. Fed. Proc. 44, 1620.