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14-745M TAO3 Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411, N47S. For use in Kinase Assays.

TAO3 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-745M
250 µg  Also available in 10 μg size (cat#14-745) and in bulk (cat# 14-745-K).
Purchase on Sigma-Aldrich

Przegląd

Replacement Information
Description
Catalogue Number14-745M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionTAO3 Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411; N47S.
References
Product Information
Presentation10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411, N47S. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
PurityPurity 84% by SDS-PAGE and Coomassie blue staining.
SourceRecombinant, human TAO3 amino acids 1-411; N47S. Expressed by baculovirus in Sf21 cells.
Specific Activity915U/mg, where one unit of TAO3 activity is defined as 1nmol phosphate incorporated into 0.8mg/ml myelin basic protein per minute at 30°C with a final ATP concentration of 100μM.
Entrez Gene Number
Gene Symbol
  • TAOK3
  • JIK
  • hKFC-A
  • MAP3K18
  • DPK
  • KDS
  • DKFZp666H245
  • FLJ31808
Protein TargetTAO3
Purification MethodNi2+/NTA-agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9H2K8 # Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
SIZE: 898 amino acids; 105406 Da
SUBUNIT: Self-associates.
SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Also localized to the peripheral cell membrane.
TISSUE SPECIFICITY: Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
PTM: Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: Q9H2K8 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain.
Molecular Weight50.2 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of TAO3.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-745) and in bulk (cat# 14-745-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
14-745M 04053252478833

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Alternative Packsize

Numer katalogowy Opis
14-745 TAO3 Protein, active, 10 µg

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Kategorie

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases