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HTS086RTA Ready-to-Assay™ H2 Histamine receptor Frozen Cells

Human H2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Ready-to-Assay™ H2 Histamine receptor Frozen Cells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-2 Entrez Gene Number: NM_022304.1
HTS086RTA
1 kit  
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

SpeciesHost CellsProtein Target
Human Chem-2H2
Description
Catalogue NumberHTS086RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ H2 Histamine receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells, resuspends them in media, and allows them to recover for 24 hours. The following day the cells are counted, plated, and assayed for calcium response.

The histamine H2 receptor is a widely distributed G-protein coupled receptor, which is important in regulating a host of physiologic actions extending from gastric acid secretion to gastrointestinal motility (Del Valle and Gantz, 1997). Histamine mediated activation of its receptor leads to signaling through both adenylate cyclase and phosphoinositide /protein kinase C second messenger systems (Hill, 1990). Antagonists for this receptor have proven to be effective therapy for acid peptic disorders of the GI tract. Certain antagonists are used in the treatment of neuropsychiatric and neurological diseases e.g. schizophrenia, Alzheimer's disease and Parkinson’s disease. Millipore’s cloned human H2-expressing cell line is made in the Chem-2 host, which supports high levels of recombinant H2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at H2.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Quality LevelMQ100
Applications
ApplicationHuman H2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-2
Protein TargetH2
Target Sub-FamilyHistamine
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
HTS086RTA 04053252821653

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Kategorie

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells