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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

03-114 Sigma-AldrichRIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal

This RIPAb+ Musashi 1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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References

Key Applications: RNA Binding Protein Immunoprecipitation (RIP), Western Blotting, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin) Research Category: Epigenetics & Nuclear Function, Neuroscience Research Sub-Category: RNA Metabolism & Binding Proteins, RNA Binding Protein (RBP), Neuronal & Glial Markers

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Species Reactivity	Key Applications
H, R	RIP, WB, FC, ICC, IP, IH(P)

Description
Catalogue Number	03-114
Brand Family	Upstate
Trade Name	RIPAb+ Upstate
Description	RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal
Overview	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Alternate Names	Musashi (Drosophila) homolog 1 musashi 1 musashi homolog 1 (Drosophila)
Background Information	In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.

References

Product Information
Format	Unpurified
Control	Includes negative control rabbit IgG antibody and control primers specific for human NUMB.
Presentation	Anti-Musashi 1 (Rabbit Monoclonal). One vial containing 50 μL of Rabbit Monoclonal Antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Store at -20°C. Normal Rabbit IgG. One vial containing 125 μg of Rabbit IgG in 125 μL of storage buffer containing 0.05% sodium azide. Store at -20°C. RIP Primers, NUMB. One vial containing 75 μL of 5 μM of each primer specific for human NUMB. Store at -20°C. FOR: AGC CAG CCC ATA CTG CTC TA REV: CGG ACG CTC TTA GAC ACC TC

Applications
Application	This RIPAb+ Musashi 1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications	RNA Binding Protein Immunoprecipitation (RIP) Western Blotting Flow Cytometry Immunocytochemistry Immunoprecipitation Immunohistochemistry (Paraffin)
Application Notes	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from SHSH-5Y cells (2 X 10E6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or 0.5 µL of Anti-Musashi 1 antibody. Precipitated proteins (lane 1: rabbit IgG, lane 2: anti-Musashi 1) and SHSH-5Y whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Musashi 1 antibody (1:2,000 dilution). Proteins were visualized using One-Step™ IP-Western kit (GenScript). Arrow indicates Musashi 1 (Please see figures). Immunocytochemistry Analysis: Representative lot data. A previous lot of this antibody was used in immunofluorescent staining of PC12 cells using Anti-Musashi 1 (Please see figures). Immunohistochemistry (Paraffin) Analysis: Representative lot data. A previous lot of this antibody was used in immunohistochemical staining of paraffin-embedded human brain using Anti-Musashi 1 (Please see figures). Western Blot Analysis: Representative lot data. SHSY5Y cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Musashi 1 (1:2,000 dilution). Proteins were visualized using a Goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Musashi 1 (~39 kDa) (Please see figures). Flow Cytometry: A 1:40 dilution of a previous lot was used in flow cytometry.

Biological Information
Immunogen	Synthetic peptide corresponding to residues on the N-terminus of human Musashi-1.
Epitope	N-terminus
Host	Rabbit
Specificity	Recognizes Musashi 1 at the N-terminus.
Isotype	IgG
Species Reactivity	Human Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_002433.1
Entrez Gene Summary	This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
Gene Symbol	MSI1
Purification Method	Unpurified
UniProt Number	O43347
UniProt Summary	FUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus By similarity. TISSUE SPECIFICITY: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. DOMAIN: The first RNA recognition motif binds more strongly to RNA compared to the second one By similarity. SEQUENCE SIMILARITIES: Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.
Molecular Weight	~39 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from SHSH-5Y cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-Musashi 1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Musashi 1-associated RNA was verified by qPCR using RIP Primers NUMB (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from SHSH-5Y cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-Musashi 1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Musashi 1-associated RNA was verified by qPCR using RIP Primers NUMB (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	10 assays
Material Package	10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context).

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Numer katalogowy	GTIN
03-114	04053252746680

Documentation

RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal MSDS

Title
Safety Data Sheet (SDS)

RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal Certificates of Analysis

Title	Lot Number
RIPAb+ Musashi 1 - 2080322	2080322
RIPAb+ Musashi 1 - 2268790	2268790
RIPAb+ Musashi 1 - NRG1761581	NRG1761581

References

Reference overview	Pub Med ID
Increased particulate phosphodiesterase 4 in the prefrontal cortex supports 5-HT4 receptor-induced improvement of object recognition memory in the rat. Guénaëlle Levallet,Maïté Hotte,Michel Boulouard,François Dauphin Psychopharmacology 202 2009 Pokaż streszczenie Serotonin receptors (5-HT4Rs) are critical to both short-term and long-term memory processes. These receptors mainly trigger the cyclic adenosine monophosphate (cAMP)/protein kinase A signaling pathway, which is regulated by cAMP phosphodiesterases (PDEs).	18712363
Inhibition of Bacillus subtilis and Listeria innocua by nisin in combination with some naturally occurring organic compounds. N A Olasupo,D J Fitzgerald,A Narbad,M J Gasson Journal of food protection 67 2004 Pokaż streszczenie The application of combined preservative factors (hurdle technology) is very effective in controlling the growth of food spoilage and foodborne pathogenic bacteria. Antimicrobial activity of nisin alone and in combination with some natural organic compounds (carvacrol, cinnamic acid, eugenol, diacetyl, and thymol) on the growth of gram-positive bacteria Bacillus subtilis and Listeria innocua was-investigated. All the organic compounds tested exhibited antimicrobial activity against the microorganisms used; however, the MICs varied between 0.8 and 15.0 mM depending on the potency of the compound or the sensitivity of the target strain. Investigation of the interaction between the organic compounds and nisin against the test organisms revealed different patterns, varying from synergistic to antagonistic. Combinations of nisin with carvacrol, eugenol, or thymol resulted in synergistic action against both test organisms. Activity of nisin and cinnamic acid together was synergistic against L. innocua, but only additive against B. subtilis. In contrast, the combination of diacetyl and nisin resulted in an antagonistic effect against both test organisms. This study highlights the potential of the combination of these compounds with nisin to inhibit pathogen growth in food.	15035380

Reference overview

Pub Med ID

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Kategorie

Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies

Life Science Research > Antibodies and Assays > Primary Antibodies

Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies

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