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14-567 Sigma-AldrichPyk2 Protein, active, 10 µg

Active, N-Terminal His6-tagged, recombinant, full-length, human Pyk2. For use in Kinase Assays.

Pyk2 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
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Description
Catalogue Number	14-567
Brand Family	Upstate
Trade Name	Upstate
Description	Pyk2 Protein, active, 10 µg
Overview	N-Terminal His6-tagged, recombinant, full-length, human Pyk2.
Alternate Names	Protein-tyrosine kinase 2-beta Calcium-dependent tyrosine kinase CADTK Calcium-regulated non-receptor proline-rich tyrosine kinase Cell adhesion kinase beta CAK-beta CAKB Focal adhesion kinase 2 FADK 2 Proline-rich tyrosine kinase 2 Related adhesion focal tyrosine kinase RAFTK

References

Product Information
Quality Level	MQ100

Applications
Application	Active, N-Terminal His6-tagged, recombinant, full-length, human Pyk2. For use in Kinase Assays.
Key Applications	Kinase Assay

Biological Information
Source	expressed by baculovirus in Sf21 insect cells
Specific Activity	For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number	NM_004103.3 NM_173174.1 NM_173175.1 NM_173176.1
Entrez Gene Summary	This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
Gene Symbol	PTK2B RAFTK FAK2 PYK2 FADK2 PTK CADTK CAKB PKB
Protein Target	Pyk2
Purification Method	Ni²⁺/NTA-agarose
Target Sub-Family	TK
UniProt Number	Q14289
UniProt Summary	FUNCTION: SwissProt: Q14289 # Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. SIZE: 1009 amino acids; 115875 Da SUBUNIT: Interacts with Crk-associated substrate (Cas), PTPNS1 and SH2D3C (By similarity). Interacts with nephrocystin, DDEF2, OPHN1L, SKAP2 and TGFB1I1. SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Interaction with nephrocystin induces the membrane-association of the kinase. TISSUE SPECIFICITY: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes. PTM: Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha- lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity (By similarity). SIMILARITY: SwissProt: Q14289 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
Molecular Weight	117.2kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	6 months at -70°C

Packaging Information
Material Size	10 µg
Material Package	Also available in 250 µg size --call for pricing and availability and reference catalog number 14-567M when ordering the 250 µg size.

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Numer katalogowy	GTIN
14-567	04053252476082

Documentation

Pyk2 Protein, active, 10 µg MSDS

Title
Safety Data Sheet (SDS)

Pyk2 Protein, active, 10 µg Certificates of Analysis

Title	Lot Number
Pyk2, active - 1623027-B	1623027-B
Pyk2, active - 1816613-A	1816613-A

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Kategorie

Life Science Research > Proteins and Enzymes > Purified Kinases

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