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539125 Protease Assay Kit

Protease Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
539125
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Detection Methods
Colorimetric

Products

Numer katalogowyOpakowanie Ilość/opak.
539125-1KIT Butelka szklana 1 kit
Description
OverviewA kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
Catalogue Number539125
Brand Family Calbiochem®
Materials Required but Not Delivered• Trichloroacetic acid (TCA), 5%
• Spectrophotometer that can accurately measure the absorbance at 492 nm
• Pipetting devices for accurate delivery of volumes required for the assay
• Cuvettes with optical properties suitable for use at 492 nm
• A constant-temperature water bath set at 37°C
• Microfuge for generating approximately 12,000 x g
References
ReferencesTwining, S.S. 1984. Anal. Biochem. 143, 30.
Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.
Product Information
Detection methodColorimetric
Form200 Tests
FormatCuvette or 96-well plate
Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
Quality LevelMQ100
Applications
Biological Information
Assay time3 h
Sample TypeBiological samples
Physicochemical Information
Sensitivity10 ng/ml
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Storage Conditions• FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light.
• Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
• Incubation buffer and assay buffer may be stored at 4°C or -20°C.
Protect from Light Protect from light
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
Specifications
Global Trade Item Number
Numer katalogowy GTIN
539125-1KIT 04055977195347

Documentation

Protease Assay Kit MSDS

Title

Safety Data Sheet (SDS) 

Protease Assay Kit Certificates of Analysis

TitleLot Number
539125

References

Przegląd literatury
Twining, S.S. 1984. Anal. Biochem. 143, 30.
Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.

User Protocols

Title
User Protocol