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PK1010 PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb

This PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr525/526).

PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PK1010
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
PK1010-50UL Ampulka plastikowa 50 ul
Description
OverviewRecognizes the ~72 kDa Syk protein phosphorylated at Tyr525/526 in human Syk or Tyr519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.
Catalogue NumberPK1010
Brand Family Calbiochem®
Application Data
Detection of human Syk, phosphorylated on Tyr525/526, by immunoblotting. Samples: Whole cell lysate from 293T cells transfected with wild-type Syk and CD8, left untreated (left lane) or treated with phosphatase (right lane). Primary antibody: PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb (Cat. No. PK1010) (1:1000). Detection: chemiluminescence.
References
ReferencesCoopman, P.J., et al. 2000. Nature 406, 742.
Turner, M., et al. 2000. Immunol. Today 21, 148.
Zang, J., et al. 2000. J. Biol. Chem. 275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev. 165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol. 9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol. 9, 309.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Positive control293T cells transfected with wild-type Syk and CD8
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (1:1000)
Immunoblotting (1:1000)
Application CommentsTyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDo not aliquot.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
PK1010-50UL 04055977227017

Documentation

PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb Certificates of Analysis

TitleLot Number
PK1010

References

Przegląd literatury
Coopman, P.J., et al. 2000. Nature 406, 742.
Turner, M., et al. 2000. Immunol. Today 21, 148.
Zang, J., et al. 2000. J. Biol. Chem. 275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev. 165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol. 9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol. 9, 309.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-October-2007 RFH
ApplicationELISA (1:1000)
Immunoblotting (1:1000)
Application Data
Detection of human Syk, phosphorylated on Tyr525/526, by immunoblotting. Samples: Whole cell lysate from 293T cells transfected with wild-type Syk and CD8, left untreated (left lane) or treated with phosphatase (right lane). Primary antibody: PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb (Cat. No. PK1010) (1:1000). Detection: chemiluminescence.
DescriptionProtein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr525/526.
BackgroundSyk is a protein tyrosine kinase that is widely expressed and plays a key role in hematopoietic cell signaling. Syk is reported to interact with immunoreceptor tyrosine-based activation motifs (ITAMs) found in the cytoplasmic domains of immune receptors. This interaction serves to couple activated immune receptors to downstream signaling events that mediate diverse cellular responses, such as proliferation, differentiation, and phagocytosis. Syk is also reported to act as a potential tumor suppressor in human breast carcinomas. Phosphorylation of Tyr525/526, located in the activation loop of human Syk, is required for Syk activity.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk
IsotypeIgG
Specieshuman, mouse
Positive control293T cells transfected with wild-type Syk and CD8
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
PreservativeNone
CommentsTyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDo not aliquot.
Toxicity Standard Handling
ReferencesCoopman, P.J., et al. 2000. Nature 406, 742.
Turner, M., et al. 2000. Immunol. Today 21, 148.
Zang, J., et al. 2000. J. Biol. Chem. 275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev. 165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol. 9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol. 9, 309.