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33-038 PI 3-Kinase Class II HTRF Assay

One-plate kit (384 well plate) of reagents for performing PI 3-Kinase (Class II) 4-step HTRF assays. Suitable for the following Kinases: PI3KC2α, PI3KC2β & PI3KC2γ.

PI 3-Kinase Class II HTRF Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
33-038
384 wells  Kit capacity: 384 wells
Purchase on Sigma-Aldrich

Przegląd

Replacement Information
Description
Catalogue Number33-038
Brand Family Upstate
Trade Name
  • HTRF
  • Upstate
DescriptionPI 3-Kinase Class II HTRF Assay
References
Product Information
Components
  • Reaction Buffer (4X)
  • DM A Detection Reagent
  • DM C Detection Reagent
  • DM D Detection Reagent
  • Phosphatidylinositol (1mM)
  • Phosphatidylserine (1mM)
  • Stop Reagent C
Detection methodHTRF
Quality LevelMQ300
Applications
ApplicationOne-plate kit (384 well plate) of reagents for performing PI 3-Kinase (Class II) 4-step HTRF assays. Suitable for the following Kinases: PI3KC2α, PI3KC2β & PI3KC2γ.
Key Applications
  • Kinase Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
Analytes Available
  • PI 3-Kinase Class II
Entrez Gene Number
Entrez Gene SummaryPhosphoinositide 3-kinase-C2 proteins generate phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. This protein belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.
Gene Symbol
  • PI3KC2α
  • PI3KC2β
  • PI3KC2γ
Target Sub-FamilyPI3K/PI4K
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions12 months at -70°C
Packaging Information
Material Size384 wells
Material PackageKit capacity: 384 wells
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
33-038 04053252374531

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Kategorie

Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Enzyme Assay Kits and Reagents