Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

482777 Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli

Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Inducible Nitric Oxide Synthase, iNOS, Mouse, Recombinant

482777
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
482777-50U Ampulka plastikowa 50 u
Description
OverviewRecombinant, mouse inducible NOS expression in E. coli.
Catalogue Number482777
Brand Family Calbiochem®
SynonymsInducible Nitric Oxide Synthase, iNOS, Mouse, Recombinant
References
ReferencesStevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will produce 1.0 nmol nitric oxide per min at 37°C, pH 7.4.
FormLiquid
FormulationIn 50 mM HEPES, 10% glycerol, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥2.9 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22-36/37/38

Harmful by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
482777-50U 04055977200645

Documentation

Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

Nitric Oxide Synthase, Inducible, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
482777

References

Przegląd literatury
Stevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-January-2011 JSW
SynonymsInducible Nitric Oxide Synthase, iNOS, Mouse, Recombinant
DescriptionRecombinant, mouse inducible NOS expression in E. coli.
FormLiquid
FormulationIn 50 mM HEPES, 10% glycerol, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions50 mM HEPES, 1 mM arginine, 1 mM magnesium acetate, 0.1 mM NADPH, 5 µM oxyhemoglobin, 12 µM tetrahydrobiopterin, 170 µM DTT, pH 7.4.
Specific activity≥2.9 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will produce 1.0 nmol nitric oxide per min at 37°C, pH 7.4.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Harmful
ReferencesStevens-Truss, R., and Marletta, M.A. 1995. Biochemistry 34, 15638.
Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
Marletta, M.A. 1994. J. Med. Chem. 37, 1899.
White, K.A., and Marletta, M.A. 1992. Biochemistry 31, 6627.
Hevel, J.M., et al. 1991. J. Biol. Chem. 266, 22789.