Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

411550 Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
411550
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
411550-500UG Butelka szklana 500 μg
Description
Catalogue Number411550
Brand Family Calbiochem®
References
ReferencesHamilton, R.G. 1993. Clin. Chem. 39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
Product Information
FormLiquid
FormulationIn PBS, 10 mg/ml BSA.
PreservativeNone
Quality LevelMQ200
Applications
Application ReferencesEnzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
Key Applications Enzyme Immunoassay
Application NotesEnzyme Immunoassay (1:1000, see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.01%
Human IgA: <0.01%
Human IgE: <0.01%

There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Biological Information
CloneHP6043
HostMouse
IsotypeIgG
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
411550-500UG 07790788060275

Documentation

Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate MSDS

Title

Safety Data Sheet (SDS) 

Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate Certificates of Analysis

TitleLot Number
411550

References

Przegląd literatury
Hamilton, R.G. 1993. Clin. Chem. 39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-March-2009 JSW
ApplicationEnzyme Immunoassay (1:1000, see application references)
DescriptionPurified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the CALBIOCHEM® technical monograph, The Human IgG Subclasses, is available free of charge.
HostMouse
CloneHP6043
IsotypeIgG
FormLiquid
FormulationIn PBS, 10 mg/ml BSA.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.01%
Human IgA: <0.01%
Human IgE: <0.01%

There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Toxicity Standard Handling
ReferencesHamilton, R.G. 1993. Clin. Chem. 39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
Application referencesEnzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.