Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCM015 Mesenchymal Stem Cell Expansion Medium (1x)

The Mesenchymal Stem Cell Expansion Medium (1x) has been optimized & validated for Stem cell culture. This medium is available in a 500mL format.

Mesenchymal Stem Cell Expansion Medium (1x) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
SCM015
500 mL  
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Key Applications
Cult
Description
Catalogue NumberSCM015
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionMesenchymal Stem Cell Expansion Medium (1x)
References
Product Information
HS Code3821 00 00
PresentationMesenchymal Stem Cell Expansion Medium is a proprietary formulation that contains fetal bovine serum.
Quality LevelMQ200
Applications
ApplicationThe Mesenchymal Stem Cell Expansion Medium (1x) has been optimized & validated for Stem cell culture. This medium is available in a 500mL format.
Key Applications
  • Stem Cell Culture
Application Notes
Thawing of Cells:

1. Do not thaw the cells until the recommended medium and appropriate plasticware and/or glassware are on hand.
2. Remove the vial of mesenchymal stem cells from liquid nitrogen and incubate in a 37°C water bath. Closely monitor until the cells are completely thawed. Maximum cell viability is dependent on the rapid and complete thawing of frozen cells. IMPORTANT: Do not vortex the cells.
3. As soon as the cells are completely thawed disinfect the outside of the vial with 70% ethanol. Proceed immediately to the next step.
4. In a laminar flow hood, use a 1 or 2 mL pipette to transfer the cells to a sterile 15 mL conical tube. Be careful to not introduce any bubbles during the transfer process.
5. Using a 10 mL pipette, slowly add dropwise 9 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C) to the 15 mL conical tube. IMPORTANT: Do not add the whole volume of medium at once to the cells. This may result in decreased cell viability due to osmotic shock.
6. Gently mix the cell suspension by slow pipeting up and down twice. Be careful to not introduce any bubbles. IMPORTANT: Do not vortex the cells.
7. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
8. Decant as much of the supernatant as possible. Steps 4-8 are necessary to remove residual cryopreservative (DMSO).
9. Resuspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C).
10. Plate the cell mixture onto a 10-cm tissue culture plate.
11. Incubate the cells at 37°C in a 5% CO2 humidified incubator.
12. The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C). Exchange with fresh medium every two to three days thereafter.
13. When the cells are approximately 80% confluent, they can be dissociated with Accutase™ and passaged or alternatively frozen for later use.

Subculturing:

1. Carefully remove the medium from the 10-cm tissue culture plate containing the confluent layer of mesenchymal stem cells.
2. Apply 3-5 mL of Accutase™ and incubate in a 37°C incubator for 3 minutes.
3. Inspect the plate and ensure the complete detachment of cells by gently tapping the side of the plate with the palm of your hand. 4. Apply 5 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C) to the plate.
5. Transfer the dissociated cells to a 15 mL conical tube.
6. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
7. Discard the supernatant
8. Apply 2 mL of Mesenchymal Stem Cell Expansion Medium to the conical tube and resuspend the cells thoroughly. IMPORTANT: Do not vortex.
9. Count the number of cells using a hemacytometer.
10. Plate the cells to the desired density into the appropriate flasks, plates or wells in Mesenchymal Stem Cell Expansion. We typically plate the cells at ~2 million cells per 10-cm plate or T75 flask.
Biological Information
Media FormLiquid
Stem Cell Type
  • Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEach lot of medium is tested for the optimal cell growth and proliferation of mesenchymal stem cells. Sterility Testing: Negative
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMesenchymal Stem Cell Expansion Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
Packaging Information
Material Size500 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
SCM015 04053252505782