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14-548M MSK1 Protein, active, 250 µg

Active, N-terminal His6-tagged recombinant human MSK1 residues 2-end, for use in Kinase Assays.

MSK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-548M
250 µg  Also available in 10 μg size (cat#14-548) and in bulk (cat# 14-548-K).
Purchase on Sigma-Aldrich

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Replacement Information
Description
Catalogue Number14-548M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMSK1 Protein, active, 250 µg
OverviewN-terminal His6-tagged recombinant human MSK1 residues 2-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged recombinant human MSK1 residues 2-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • RPS6KA5
  • RSKL
  • RLPK
  • MGC1911
  • MSK1
  • MSPK1
Protein TargetMSK1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O75582 # Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
COFACTOR: Magnesium.
SIZE: 802 amino acids; 89865 Da
SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Predominantly nuclear. Partially cytoplasmic.
TISSUE SPECIFICITY: Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
PTM: Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C- terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
SIMILARITY: SwissProt: O75582 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains.
MISCELLANEOUS: Enzyme activity requires the presence of both kinase domains.
Molecular Weight94kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Crosstide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -20°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-548) and in bulk (cat# 14-548-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
14-548M 04053252471599

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Numer katalogowy Opis
14-548 MSK1 Protein, active, 10 µg

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Kategorie

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases