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48-618MAG MILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.

MILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
48-618MAG
96-well plate  96-well plate
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Tabela kluczowych gatunków

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Akt (Total) Akt (Ser473) H, M, RMplexLuminex xMAP
Description
Catalogue Number48-618MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay
OverviewThe MILLIPLEX® Total/Phospho Akt (48-618MAG) and Total/Phospho ERK (Catalog # 48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • Akt (Total)
  • Akt (Ser473)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
48-618MAG 04053252859700