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112084 HEMATOGNOST Fe™

staining kit for the detection of free ionic iron (Fe³⁺) in cells

HEMATOGNOST Fe™ MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
112084
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Numer katalogowyOpakowanie Ilość/opak.
1120840001 Pudelko kartonowe 4 x 250 ml
Description
Catalogue Number112084
DescriptionHEMATOGNOST Fe™
OverviewHEMATOGNOST Fe™ - staining kit for the detection of free ionic iron (Fe3+) in cells, is used for human-medical cell diagnosis and serves the purpose of the hematological and histological investigation of sample material of human origin. HEMATOGNOST Fe™ is a staining kit for performing the so-called Prussian blue (Berlin blue) reaction in hematological and histological specimen material. This staining kit contains all the reagents necessary for the detection of free ionic iron. In the Prussian blue reaction, ionic iron (Fe3+) not bound to the heme structure reacts with potassium hexacyanoferrate(II) in hydrochloric solution. It precipitates as an insoluble complex salt in the blood, bone-marrow, or tissue cells and thus localizes free cellular iron: 4 Fe3+ + 3 K4Fe(CN)6 = Fe4[Fe(CN)6]3 + 12 K+
As a measure to achieve the best possible visual differentiation of the iron deposits in the cytoplasma, this is counterstained with nuclear fast red solution (Nuclear fast red-aluminum sulfate solution 0.1% (Cat. no.100121), resulting in a tender pink color. If further, more detailed information on the cell morphology is required, counterstaining with any of the other familiar “classical” methods such as Giemsa, (Giemsa, Cat. no. 109204), May-Grünwald (May-Grünwald eosin methylene blue solution, Cat.no 101424), hemalum (Mayer's hemalum solution, Cat. no.109249), etc. can also be employed.
The Prussian blue reaction can also be used to stain specimens already stained by one of these classical procedures for the additional detection of iron. Repeat counterstaining is not necessary in this case.
The staining kit is sufficient for 6 - 8 stainings with up to 16 slides. The staining kit is developed and registered as CE / IVD product for applications in diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationstaining kit for the detection of free ionic iron (Fe³⁺) in cells
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H290: Może powodować korozję metali.
H318: Powoduje poważne uszkodzenie oczu.
Precautionary Statement(s)P234: Przechowywać wyłącznie w oryginalnym opakowaniu.
P280: Stosować ochronę oczu/ ochronę twarzy.
P305 + P351 + P338: W PRZYPADKU DOSTANIA SIĘ DO OCZU: Ostrożnie płukać wodą przez kilka minut. Wyjąć soczewki kontaktowe, jeżeli są i można je łatwo usunąć. Nadal płukać.
P390: Usunąć wyciek, aby zapobiec szkodom materialnym.
Signal WordNiebezpieczeństwo
Storage class8B Niepalne, żrące materiały niebezpieczne
Safety Information
Product Usage Statements
Storage and Shipping Information
StoragePrzechowywać w +15°C do +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 3316 Chemie-Testsatz, 9, II
Declaration (transport by air) IATA-DGRUN 3316 CHEMICAL KIT, 9, II
Declaration (transport by sea) IMDG-CodeUN 3316 CHEMICAL KIT, 9, II
Supplemental Information
Specifications
Potassium hexacyanoferrate II (Reagent 1)4.5 - 5.5 %
Hydrochloric acid (Reagent 2)4.5 - 5.5 %
Density (Reagent 3 d 20 °C/4 °C)1.040 - 1.060
Suitability for microscopypasses test
Global Trade Item Number
Numer katalogowy GTIN
1120840001 04022536139234

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Kategorie

IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Kits
IVD/OEM Materials & Reagents > Microscopy > Hematology > Cytochemical Reagents