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14-569M ErbB4 Protein, active, 250 µg

Active, N-Terminal His6-tagged, recombinant, human ErbB4 amino acids 706-991, for use in Kinase Assays.

ErbB4 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-569M
250 µg  Also available in 10 μg size (cat#14-569) and in bulk (cat# 14-569-K).
Purchase on Sigma-Aldrich

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Replacement Information
Description
Catalogue Number14-569M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionErbB4 Protein, active, 250 µg
OverviewN-Terminal His6-tagged, recombinant, human ErbB4 amino acids 706-991
Alternate Names
  • Receptor Tyrosine-protein kinase erbB-4
  • p180erbB4
  • Tyrosine kinase-type cell surface receptor HER4
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-Terminal His6-tagged, recombinant, human ErbB4 amino acids 706-991, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
Gene Symbol
  • ERBB4
  • HER4
Protein TargetErbB4
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P21860 # Binds and is activated by neuregulins and NTAK.
SIZE: 1342 amino acids; 148098 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 and PA2G4.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Epithelial tissues and brain.
DOMAIN: SwissProt: P21860 The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
PTM: Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (By similarity).
DISEASE: SwissProt: P21860 # Overexpressed in a subset of human mammary tumors. & Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
Molecular Weight36.1kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-569) and in bulk (cat# 14-569-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
14-569M 04053252280771

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Kategorie

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases