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324699 Elastase Substrate IV, Colorimetric - Calbiochem

A specific substrate for pancreatic elastase.

Elastase Substrate IV, Colorimetric - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
324699
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Przegląd

Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
324699-5MG Ampulka plastikowa 5 mg
Description
OverviewA specific substrate for pancreatic elastase (km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase, Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.
Catalogue Number324699
Brand Family Calbiochem®
References
ReferencesLargman, L. 1983. Biochemistry 22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry 19, 468.
Product Information
ATP CompetitiveN
FormWhite to off-white lyopilized solid
Formulation45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
Hill FormulaC₂₅H₃₄N₆O₉
Chemical formulaC₂₅H₃₄N₆O₉
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetA specific substrate for pancreatic elastase
Primary Target IC<sub>50</sub>km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceSuc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
324699-5MG 04055977215915

Documentation

Elastase Substrate IV, Colorimetric - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Elastase Substrate IV, Colorimetric - Calbiochem Certificates of Analysis

TitleLot Number
324699

References

Przegląd literatury
Largman, L. 1983. Biochemistry 22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry 19, 468.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-June-2008 RFH
DescriptionA specific substrate for pancreatic elastase (Km = 100 µM; Kcat/Km = 35,000 s-1 M-1 for rat pancreatic elastase; Km = 30 µM; Kcat/Km = 351,000 s-1 M-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.

Suggested protocol: see Largman, C. (1983)
FormWhite to off-white lyopilized solid
Formulation45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
Chemical formulaC₂₅H₃₄N₆O₉
Peptide SequenceSuc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)
Purity≥95% by HPLC
SolubilityEthanol (1 mg/ml) or H₂O
Storage Protect from moisture
Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Toxicity Standard Handling
ReferencesLargman, L. 1983. Biochemistry 22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry 19, 468.