ECM Proteins and Coated Plates

ECM Options

Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. Studies show that anchorage dependent cells growing on ECM undergo more efficient plating, have a higher proliferation rate, reach a higher density, and require lower serum and growth factor concentrations—demonstrating enhanced differentiation potential.

Millipore offers a wide variety of ECM proteins and coated cultureware to meet the individual needs of your cell line. Our ECM Cell Culture Optimization Array can help you determine the best coating and concentration for your particular cell type. Once you know what you need, you can choose from the convenience of precoated cultureware or the flexibility of making your own coatings with our ECM proteins and plates. No matter what your research demands, we have a format to meet your needs.

ECM Proteins

From the most commonly used proteins to more specialized matrices, Millipore has the substrate you need. Please see our list below for the most up-to-date selection—we are constantly adding new products to support your research.

Millicoat™ Extracellular Matrix (ECM)-Coated Plasticware

Millipore now offers pre-coated multiwell plates in 6- and 24-well formats. Coatings include collagen, fibronectin, and poly-D-lysine. Pre-coated products offer a great advantage to researchers. There is no lengthy coating process, plates are coated by a consistent process and they are always available when needed.

Millicoat™ ECM Coated Strips

For convenience and flexibility in designing assays, try our Millicoat ECM coated strips, provided as 12 removable 8-well strips in a plate frame. The wells in rows A-G are precoated with an ECM protein and row H is coated with BSA to serve as a negative control.

Optimization Array

The ECM Cell Culture Optimization Array is the first commercially-available tool of its kind to enable researchers to not only quickly identify the best ECM protein for their cell culture environment, but also pinpoint the concentration needed to achieve optimal cell growth conditions.

Tissue Culture Treated Plates

Tissue culture treated plates offer a surface to which most adherent cells can attach and proliferate. The 6-, 12-, and 24-well formats provide users flexibility to run multiple samples simultaneously. These plates can be easily prepared for SEM and TEM , and are compatible with cellular and fluorescent staining procedures. These plates can be used independently for general cell culture applications, or as receiver plates for membrane-based Millicell^® inserts. All plates are individually wrapped and sterilized.