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70526 Clonables™ Ligation/Transformation Kit - Novagen

Simple, reproducible ligation and transformation—in as little as 23 minutes

Clonables™ Ligation/Transformation Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70526
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
70526-3 Ampulka plastikowa 11 rxn
Description
OverviewThe Clonables™ Kit enables convenient, dependable, high-efficiency ligation and transformation of any compatible DNA ends. The kit features a unique, universal Ligation Premix, containing ligase, buffer, and cofactors, which supports ligation of any type of DNA cohesive or blunt ends in a 15-minute reaction. Ligated DNA is transformed into NovaBlue Singles™ Competent Cells, which use a streamlined protocol that takes less than 8 minutes for ampicillin-resistant plasmids and 38 minutes for other antibiotic-resistant plasmids. This kit can be used with a variety of cloning vectors and is compatible with any type of DNA end, without altering the ends or desired cloning junctions.

The Clonables Kit contains sufficient reagents to perform 11 ligation and transformation reactions, and includes a control vector and insert mix to verify performance.
Catalogue Number70526
Brand Family Novagen®
Features and benefits
  • Fast! 15-minute ligation, 8-minute transformation with ampicillin selection
  • Ligation components premixed to decrease pipetting steps and increase reliability
  • One reaction, optimized for cohesive ends, single base overhangs, and blunt ends
  • Single-use competent cells eliminate need to aliquot, freeze/thaw, or waste partially used vials
  • Compatible with PCR buffer, TE, restriction enzyme buffer, or End Conversion Mix
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    References
    Product Information
    Components
    Quality LevelMQ100
    Applications
    Biological Information
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Dry Ice Only
    Toxicity Multiple Toxicity Values, refer to MSDS
    Storage ≤ -70°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Numer katalogowy GTIN
    70526-3 04055977273595

    Documentation

    Clonables™ Ligation/Transformation Kit - Novagen Certificates of Analysis

    TitleLot Number
    70526

    Brochure

    Title
    High fidelity gene amplification

    Citations

    Tytuł
  • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
  • Bratislav Janjic, et al. (2006) Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. Journal of Immunology 177, 2717-2727.
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    User Protocols

    Title
    TB233 Clonables™ Kit