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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

17-10267 Sigma-AldrichChIPAb+™ FOXA1 Antibody

This ChIPAb+ FOXA1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

ChIPAb+™ FOXA1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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Key Applications: Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors

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Tabela kluczowych gatunków

Species Reactivity	Key Applications
H, M	WB, ICC, ChIP

Description
Catalogue Number	17-10267
Trade Name	ChIPAb+ Upstate
Description	ChIPAb+™ FOXA1 Antibody
Overview	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+™ FOXA1 set includes the FOXA1 antibody, a Normal Rabbit IgG, and control primers which amplify a 138 bp region of ChIP Primers, Mouse Hnf4α enhancer. The FOXA1 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of FOXA1-associated chromatin.
Alternate Names	Forkhead box protein A1 Hepatocyte nuclear factor 3-alpha HNF-3A Transcription factor 3A TCF-3A
Background Information	HNF-3A, Hepatocyte nuclear factor 3-alpha (also called FOXA1) is member of the Forkhead family of winged-helix transcription factors. This protein family has around 50 known transcription factors and play an important role in the regulation of cell differentiation, organogenesis, and gene expression. High levels of expression of HNF-3A has been observed in tumors arising from both the prostate and breast. Here it is believed this protein interacts with the androgen receptor in prostate tumors and the estrogen receptor α in breast tumors. In the case of ERα, HNF-3A interacts with cis-regulatory regions in heterochromatin and enhances the interaction of ERα with chromatin.

References

Product Information
Format	Affinity Purified
Control	Includes normal rabbit IgG and primers specific for Mouse Hnf4α enhancer.
Presentation	Anti-FOXA1 (Rabbit Polyclonal). One vial containing 50 µL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C. Concentration: 0.35 mg/mL Normal Rabbit IgG. One vial containing 125 µg Rabbit IgG in 125 µL storage buffer containing 0.05% sodium azide. Store at -20°C. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75 μL of 5 μM of each primer specific for Mouse Hnf4α enhancer. Store at -20°C. FOR: TTC CAG CTG CCT TTA TCT CCC TGT REV: TCT CCA CAC ATG TCC AGC AGC CT
Quality Level	MQ100

Applications
Application	This ChIPAb+ FOXA1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications	Western Blotting Immunocytochemistry Chromatin Immunoprecipitation (ChIP)
Application Notes	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µl of either normal rabbit IgG, or 2 µl Anti-FOXA1 (Part No.CS207368) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of FoxA1 associated DNA fragments was verified by qPCR using ChIP Primers, Mouse Hnf4α enhancer as a positive locus, and mouse Hnf4α promoter as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blotting Analysis: Representative lot data. Human pancreas tissue lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-FOXA1 (0.5 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. (Figure 3) Arrow indicates FOXA1 (~51 kDa). Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3 and HeLa cells using Anti-HNF-3A/FOXA1 (Red). Actin filaments have been labeled with Alexa Fluor® 488 Dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains nucleus. (Figure 4)

Biological Information
Immunogen	Recombinant protein corresponding to the entire protein of human FOXA1
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes HNF-3A/FOXA1.
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_004487
Entrez Gene Summary	This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq].
Gene Symbol	FOXA1 HNF-3-alpha HNF-3A HNF3A TCF3A MGC33105
Purification Method	Affinity Purfied
UniProt Number	P55317
UniProt Summary	FUNCTION: Transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. SUBCELLULAR LOCATION: Nucleus. SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain.
Molecular Weight	~51 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Chromatin Immunoprecipitation: Representative lot data Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit IgG,or 2 µL Anti-FOXA1 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of FOXA1- associated DNA fragments was verified by qPCR using ChIP Primers, Mouse Hnf4α enhancer (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Chromatin Immunoprecipitation:
Representative lot data
Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit IgG,or 2 µL Anti-FOXA1 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of FOXA1- associated DNA fragments was verified by qPCR using ChIP Primers, Mouse Hnf4α enhancer (Figure 1).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	25 assays
Material Package	25 assays per set. Recommended use: 2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Numer katalogowy	GTIN
17-10267	04053252439445

Documentation

ChIPAb+™ FOXA1 Antibody MSDS

Title
Safety Data Sheet (SDS)

ChIPAb+™ FOXA1 Antibody Certificates of Analysis

Title	Lot Number
ChIPAb+ FOXA1 - 2197301	2197301
ChIPAb+ FOXA1 - Q1999626	Q1999626
ChIPAb+ FOXA1	2837840
ChIPAb+ FOXA1 - 3202461	3202461
ChIPAb+ FOXA1 - 4033850	4033850
ChIPAb+ FOXA1 -2565382	2565382
ChIPAb+ FOXA1 -2740908	2740908
ChIPAb+ FOXA1 -2821623	2821623
ChIPAb+ FOXA1 Polyclonal Antibody/Primer Set	2893741

Brochure

Title
Advance your Epigenetics Research
New Products: Volume 3, 2012

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Kategorie

Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies

Life Science Research > Antibodies and Assays > Primary Antibodies

Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies

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