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219467 Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells

Cathepsin V, His•Tag®, is a recombinant human procathepsin V (aa 18-334) fused at the N-terminus to a CD33 signal sequence (aa 1-16) & at C-terminus to a His•Tag® sequence & expressed in NSO cells.

Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Cathepsin L2, Cathepsin U

219467
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Przegląd

Replacement Information

Products

Numer katalogowyOpakowanie Ilość/opak.
219467-10UG Ampulka plastikowa 10 μg
Description
OverviewRecombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.
Catalogue Number219467
Brand Family Calbiochem®
SynonymsCathepsin L2, Cathepsin U
References
ReferencesTolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
Turk, V., et al. 2001. EMBO J. 20, 4629.
Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
Bromme, D., et al. 1999. Biochemistry 38, 2377.
Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
Santamaria, I., et al. 1998. Cancer Res. 58, 1624.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
EC number3.4.22.43
FormFrozen liquid
FormulationIn 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥300 units/µg
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsEndotoxins: ≤1.0 EU/µg Cathepsin V
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsTo activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
219467-10UG 04055977218497

Documentation

Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells MSDS

Title

Safety Data Sheet (SDS) 

Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells Certificates of Analysis

TitleLot Number
219467

References

Przegląd literatury
Tolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
Turk, V., et al. 2001. EMBO J. 20, 4629.
Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
Bromme, D., et al. 1999. Biochemistry 38, 2377.
Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
Santamaria, I., et al. 1998. Cancer Res. 58, 1624.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-November-2008 RFH
SynonymsCathepsin L2, Cathepsin U
DescriptionRecombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.
FormFrozen liquid
FormulationIn 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.
Concentration Label Please refer to vial label for lot-specific concentration
EC number3.4.22.43
Purity≥95% by SDS-PAGE
ContaminantsEndotoxins: ≤1.0 EU/µg Cathepsin V
Specific activity≥300 units/µg
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsTo activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesTolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
Turk, V., et al. 2001. EMBO J. 20, 4629.
Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
Bromme, D., et al. 1999. Biochemistry 38, 2377.
Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
Santamaria, I., et al. 1998. Cancer Res. 58, 1624.