Benzonase® Nuclease and rLysozyme™ Solution for Extraction

Remove nucleic acids from and reduce viscosity of protein extracts with Benzonase^® Nuclease, a genetically engineered endonuclease from Serratia marcescens. It eliminates all forms of DNA and RNA (single-stranded, double-stranded, circular or linear) more efficiently than DNase I without affecting proteins. The enzyme is functional over a wide range of conditions and possesses an exceptionally high specific activity. Adding rLysozyme™ Solution, a highly purified recombinant lysozyme, which degrades bacterial cell walls, enhances extraction efficiency, especially for larger proteins. rLysozyme™ Solution exhibits 250 times higher specific activity than chicken egg white lysozyme.

Features & Benefits

• Increase protein extraction efficiency and facilitate downstream processing of protein extracts with the combined activities of rLysozyme™ Solution and Benzonase^® Nuclease
  
• Effectively reduce viscosity and remove nucleic acids from protein solutions
  
• Effective over a wide range of conditions and has an exceptionally high specific activity
  
• Convenient: Available in two purity grades (ultrapure >99% and pure >90%) and in two concentrations (standard at 25 U/μL and high concentration, HC, 250 U/μL)
  
• Versatile: Compatible with a variety of lysis reagents such as BugBuster® and CytoBuster™ reagents to eliminate viscosity and increase protein yields

Applications

Elimination of Nucleic Acids and Viscosity From Recombinant Proteins, Enhanced Protein Purification, Increased Gel Resolution, Prevention of Cell Clumping