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14-773M BTK (E41K) Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.

14-773M
250 µg  Also available in 10 μg size (cat#14-773) and in bulk (cat# 14-773-K).
Purchase on Sigma-Aldrich

Przegląd

Replacement Information
Description
Catalogue Number14-773M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionBTK (E41K) Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant, full-length, human BTK
Background InformationSpecific mutations within the pleckstrin homology (PH) domain of BTK results in human X-linked agammaglobulinemia (XLA). The E41K substitution, identified through random mutagenesis, is a PH domain gain-of-function mutation that has been shown to be associated with increased membrane localization and tyrosine phosphorylation of BTK. Using animal models, this mutation has been shown to arrest the development of immature B cells and to act as a tumour suppressor in B-cell linker protein (BLNK/SLP-65)-deficient hosts. (Li, .et al., Immunity,(1995);2:451-460 and Baraldi, E., et al., Structure,(1999);7:449-460).
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • BTK
  • ATK
  • BPK
  • AGMX1
Protein TargetBTK
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
Molecular Weight78.4kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of LKBtide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-773) and in bulk (cat# 14-773-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
14-773M 04053252324017

Documentation

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Alternative Packsize

Numer katalogowy Opis
14-773 BTK (E41K) Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Kategorie

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases