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EI3 Antipain

Antipain MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
EI3
100 mg  
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Replacement Information
Description
Catalogue NumberEI3
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAntipain
OverviewAntipain is a serine and cysteine reversible protease inhibitor. It inhibits papain and trypsin. Plasmin is inhibited to a small extent as well. More specific than leupeptin for papain and trypsin.

Sequence:

[(S)-1-Carboxy-2-Phenylethyl]-Carbamoyl-L-Arg-L-Val-Argininal-HCL-2H2O.

M.W: 604.71

Working solutions: 1-100μM.

Specific Activity: Varies with lot, IC50 is approximately 0.20μg/mL (papain/casein, pH 7.4 37°C).

Stable for several hours. Stock solutions of 10mM in water or buffer are stable for 1 week at 4ºC, 1 month at -20ºC. Also soluable in MeOH or DMSO.

5mg/827 microliters of water = 10mM solution. Dilute 1:100 for a 100μM solution.

Source: Microbial origin. Not synthetically made.
References
Product Information
PresentationLyophilized no preservatives.

Properties: Soluble in H2O, methanol and DMSO (Stock solution: 10 mM). Stable at -20° C.

Mechanism of action: Formation of a hemicetal adduct between the aldehyde group of the inhibitor and the active serine of the proteinase.

Inhibition spectrum: Inhibits papain, trypsin and plasmin to a lesser extent. More specific for papain and trypsin than leupeptin. The inhibitory potency of antipain is 100-fold higher than that of elastatinal.

Pale yellow powder; material is isolated from cultured media of microorganisms. As with many of these compounds, they are too inherently unstable to allow for high standards of purity. The homogeneity of these compounds cannot be guaranteed except for their specific inhibitory activities.
Quality LevelMQ100
Applications
Key Applications
  • Inhibits Activity/Function
Application NotesProteinase inhibitor; Inhibits papain and trypsin. Plasmin is inhibited to a small extent.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancepH 1% aqueous solution: 5.2
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain dry at -20ºC for up to 18 months. Store reconstituted product in aliquots at -20ºC for up to 9 months. Do not thaw and refreeze.
Packaging Information
Material Size100 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
EI3 04053252508844

Documentation

Antipain MSDS

Title

Safety Data Sheet (SDS) 

Antipain Certificates of Analysis

TitleLot Number
ANTIPAIN PROTEASE INHIBITOR 3084128
ANTIPAIN PROTEASE INHIBITOR 2971062
ANTIPAIN PROTEASE INHIBITOR 2905625
ANTIPAIN PROTEASE INHIBITOR 2470385
ANTIPAIN PROTEASE INHIBITOR - 2088955 2088955
ANTIPAIN PROTEASE INHIBITOR - 2278482 2278482
ANTIPAIN PROTEASE INHIBITOR - 3207642 3207642
ANTIPAIN PROTEASE INHIBITOR - 3284295 3284295
ANTIPAIN PROTEASE INHIBITOR - 3385781 3385781
ANTIPAIN PROTEASE INHIBITOR - 3482807 3482807
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References

Reference overviewPub Med ID
Antipain, a new protease inhibitor isolated from actinomycetes. {original article}
Suda, H., et al.
J. Antibiotics (Tokyo), 25:263-266 (1972)  1971

4559651 4559651
Structure of antipain, a new Sakaguchi-positive product of streptomyces {Structure}
Umezawa, S et al.
J. Antibiotics (Tokyo), 25(4):267-270 (1972)  1971

5052959 5052959

Data Sheet

Title
ANTIPAIN PROTEASE INHIBITOR