Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE1472 Anti-phospho eIF4B Antibody (Ser422)

Anti-phospho eIF4B Antibody (Ser422) | ABE1472 is an antibody against phospho eIF4B Antibody for use in Western Blotting.

Anti-phospho eIF4B Antibody (Ser422) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: EIF4B UniProt Number: P23588
ABE1472
100 µg  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1472
DescriptionAnti-phospho eIF4B Antibody (Ser422)
Alternate Names
  • Eukaryotic translation initiation factor 4B
  • eIF-4B
Background InformationEukaryotic translation initiation factor 4B (eIF-4B), encoded by the gene name ELF4B, is an RNA-binding protein that is involved in translation of RNA molecules and consequent protein synthesis. ELF4B is required for the binding of mRNA to ribosomes. ELF4B also functions in close association with EIF4-F and EIF4-A. ELF4B has been demonstrated to bind near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. ELF4B promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. ELF4B contains multiple domains, including an N-terminal RNA recognition motif (RRM) and a C-terminal Arginine Rich Motif (ARM) that facilitate RNA binding. eIF4B may play a specific role in the assembly of the 48S initiation complex. Its translational activity is enhanced by phosphorylation of its ser406 and ser422 residues via the Ras-MAPK and PI3-K/mTOR pathways. eIF4B specially targets RNA molecules involved in cell proliferation and survival in response to signals converging from these pathways.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho eIF4B Antibody (Ser422) | ABE1472 is an antibody against phospho eIF4B Antibody for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human phospho eIF4B (Ser422) near the C-terminus.
EpitopeNear C-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine, Zebrafish based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EIF4B
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~75 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected phospho eIF4B (Ser422) in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ABE1472 04055977123753