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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

07-838 Sigma-AldrichAnti-phospho-IR (Tyr972) Antibody

Anti-phospho-IR (Tyr972) Antibody detects level of phospho-IR (Tyr972) & has been published & validated for use in WB.

Anti-phospho-IR (Tyr972) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling Gene Symbol: INSR, CD220, HHF5, IR UniProt Number: P06213

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Tabela kluczowych gatunków

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	07-838
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-IR (Tyr972) Antibody
Alternate Names	Insulin Receptor CD220

References

Product Information
Format	Affinity Purified
Presentation	100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide) and 50% glycerol.
Quality Level	MQ100

Applications
Application	Anti-phospho-IR (Tyr972) Antibody detects level of phospho-IR (Tyr972) & has been published & validated for use in WB.
Key Applications	Western Blotting

Biological Information
Immunogen	A linear peptide corresponding to amino acid region encompassing the human, mouse and rat phospho-IR (pTyr972).
Host	Rabbit
Specificity	Recognizes phospho-IR (β-subunit) (pTyr972).
Isotype	IgG
Species Reactivity	Human Mouse
Species Reactivity Note	Predicted to cross-react with rat based on sequence homology (100%), but have not been tested.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000208.2 NM_001079817.1
Entrez Gene Summary	After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	INSR CD220 HHF5 IR
Modifications	Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	P06213
UniProt Summary	FUNCTION: SwissProt: P06213 # This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. Mediates the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). Can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1. SIZE: 1382 amino acids; 156307 Da SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Binds SH2B2. Interacts with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. The sequences surrounding the phosphorylated NPXY motif contribute differentially to either IRS1 or SHC1 recognition. Interacts with the SH2 domains of the 85 kDa regulatory subunit of PI3K (PIK3R1) in vitro, when autophosphorylated on tyrosine residues. Interacts with SOCS7. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Isoform Long and isoform Short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin. Isoform Short is expressed also in the spleen and lymphoblasts. PTM: After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane. & Autophosphorylated on tyrosine residues in response to insulin. & Phosphorylation of Tyr-999 is required for IRS1- and SHC1- binding. DISEASE: SwissProt: P06213 # Defects in INSR are the cause of insulin resistance (Ins resistance) [MIM:125853]. & Defects in INSR are the cause of Rabson-Mendenhall syndrome [MIM:262190]; also known as Mendenhall syndrome. It is a severe insulin resistance syndrome characterized by insulin- resistant diabetes mellitus with pineal hyperplasia and somatic abnormalities. Typical features include coarse, senile-appearing facies, dental and skin abnormalities, abdominal distension, and phallic enlargement. Inheritance is autosomal recessive. & Defects in INSR are the cause of leprechaunism [MIM:246200]; also known as Donohue syndrome. Leprechaunism represents the most severe form of insulin resistance syndrome, characterized by intrauterine and postnatal growth retardation and death in early infancy. Inheritance is autosomal recessive. & Defects in INSR may be associated with noninsulin- dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. & Defects in INSR are the cause of familial hyperinsulinemic hypoglycemia 5 (HHF5) [MIM:609968]. Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. & Defects in INSR are the cause of insulin-resistant diabetes mellitus with acanthosis nigricans type A (IRAN type A) [MIM:610549]. This syndrome is characterized by the association of severe insulin resistance (manifested by marked hyperinsulinemia and a failure to respond to exogenous insulin) with the skin lesion acanthosis nigricans and ovarian hyperandrogenism in adolescent female subjects. Women frequently present with hirsutism, acne, amenorrhea or oligomenorrhea, and virilization. This syndrome is different from the type B that has been demonstrated to be secondary to the presence of circulating autoantibodies against the insulin receptor. SIMILARITY: SwissProt: P06213 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain.
Molecular Weight	155 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 2 years at -20°C from date of shipment. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Numer katalogowy	GTIN
07-838	04053252622496

Documentation

Anti-phospho-IR (Tyr972) Antibody MSDS

Title
Safety Data Sheet (SDS)

Anti-phospho-IR (Tyr972) Antibody Certificates of Analysis

Title	Lot Number
Anti-phospho-IR (Tyr972) (Affinity purified Rabbit polyclonal IgG)	2981108A
Anti-phospho-IR (Tyr972) (Affinity purified Rabbit polyclonal IgG)	3082608
Anti-phospho-IR (Tyr972) - 3385875	3385875
Anti-phospho-IR (Tyr972) - 3447531	3447531
Anti-phospho-IR (Tyr972) - 3455143	3455143
Anti-phospho-IR (Tyr972) - 3839126	3839126
Anti-phospho-IR (Tyr972) - 3892562	3892562
Anti-phospho-IR (Tyr972) - 3947083	3947083
Anti-phospho-IR (Tyr972) - 4018502	4018502
Anti-phospho-IR (Tyr972) - 4021660	4021660

References

Reference overview	Pub Med ID
Preparation, crystallization and preliminary X-ray analysis of threonine synthase from Streptococcus mutans. De-Wei Tang,Lan-Fen Li,Ya-Mei Yu,Xiang-Yu Liu,Xiao-Dong Su,Xiaojun Zhao,Yu-He Liang Protein and peptide letters 14 2007 Pokaż streszczenie The thrC gene of Streptococcus mutans encodes threonine synthase, which is a potential target for drug design. To study the structure and function of the enzyme, the thrC gene was amplified from Streptococcus mutans genomic DNA and cloned into the expression vector pET28alpha. The protein was expressed in Escherichia coli in soluble form and purified to homogeneity. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method. The crystal diffracted to 2.5 A and belonged to space group P3(1) or P3(2), with unit cell parameters a=b=60.39 A, c=118.62 A.	17979829

Reference overview

Pub Med ID

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Kategorie

Life Science Research > Antibodies and Assays > Primary Antibodies

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