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07-820 Anti-phospho-EGFR (Tyr845) Antibody

07-820
100 µL  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IF, IHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-820
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-EGFR (Tyr845) Antibody
References
Product Information
FormatAffinity Purified
Control
  • EGF stimulated A431 cells
PresentationAffinity Purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho-EGFR (Tyr845) Antibody is validated for use in IC, IF, IH, WB for the detection of phospho-EGFR (Tyr845).
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr845) in RIPA lysates from EGF stimulated A431 cells.
Biological Information
ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr845)
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes phospho-EGFR (Tyr845).
Species Reactivity
  • Human
Species Reactivity NotePredicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EGFR
  • ERBB1
  • ERBB
  • mENA
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
Molecular Weight185 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
07-820 04053252361890

Documentation

Anti-phospho-EGFR (Tyr845) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-EGFR (Tyr845) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-EGFR (Tyr845) (Affinity purified Rabbit polyclonal IgG) 3076534
Anti-phospho-EGFR (Tyr845) (Affinity purified Rabbit polyclonal IgG) - 2428433 2428433
Anti-phospho-EGFR (Tyr845) (Affinity purified Rabbit polyclonal IgG) 2943153
Anti-phospho-EGFR (Tyr845) (Affinity purified Rabbit polyclonal IgG) - 2251732 2251732
Anti-phospho-EGFR (Tyr845) (Affinity purified Rabbit polyclonal IgG) - 3291277 3291277
Anti-phospho-EGFR (Tyr845) (Affinity purified Rabbit polyclonal IgG) - DAM1493393 DAM1493393
Anti-phospho-EGFR (Tyr845) - 1976900 1976900
Anti-phospho-EGFR (Tyr845) - 4087564 4087564
Anti-phospho-EGFR (Tyr845) - DAM1462553 DAM1462553
Anti-phospho-EGFR (Tyr845) - DAM1518945 DAM1518945

References

Reference overviewApplicationPub Med ID
Essential role for epidermal growth factor receptor in glutamate receptor signaling to NF-kappaB.
Sitcheran, R; Comb, WC; Cogswell, PC; Baldwin, AS
Molecular and cellular biology  28  5061-70  2008

Pokaż streszczenie
Western Blotting18541671 18541671
Changes in retinal cell fate induced by overexpression of EGF receptor
Lillien, L.
Nature, 377:158-62 (1995)  1994

Immunohistochemistry (Tissue)7675083 7675083
Amphiregulin induces tyrosine phosphorylation of the epidermal growth factor receptor and p185erbB2. Evidence that amphiregulin acts exclusively through the epidermal growth factor receptor at the surface of human epithelial cells.
Johnson, G R, et al.
J. Biol. Chem., 268: 2924-31 (1993)  1992

Pokaż streszczenie
7679104 7679104
Polymorphonuclear leukocytes-mediated lysis of A431 cells induced by IgG1 mouse anti-epidermal growth factor receptor monoclonal antibodies.
Kawamoto, T, et al.
In Vitro Cell. Dev. Biol., 28A: 782-6 (1992)  1992

Pokaż streszczenie
1483969 1483969
Cytotoxic properties of DAB486EGF and DAB389EGF, epidermal growth factor (EGF) receptor-targeted fusion toxins.
Shaw, J P, et al.
J. Biol. Chem., 266: 21118-24 (1991)  1991

Pokaż streszczenie
1939154 1939154

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