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NA46 Anti-p300 Mouse mAb (NM-11)

This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

Anti-p300 Mouse mAb (NM-11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NA46
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
H, RMMonoclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
NA46-100UG Ampulka plastikowa 100 μg
Description
OverviewRecognizes the p300 protein in A431, HeLa, and ML-1 cells.
Catalogue NumberNA46
Brand Family Calbiochem®
References
ReferencesDallas, P.B., et al. 1997. Hybridoma 16, 273.
<→EMD:if(output-is PDS)→> Shisler, J., et al. 1996. J. Virol. 70, 68.
Wang, H.G., et al. 1995. J. Virol. 69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene 9, 1733.
Eckner, R., et al. 1994. Genes Dev. 8, 869.
Abraham, S.E., et al. 1993. Oncogene 8, 1639.
Wang, H.G., et al. 1993. J. Virol. 67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology 186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol. 11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol. 11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol. 8, 3955.
Yee, S. and Branton, P., 1985. Virology 147, 142. <→EMD:endif()→>
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Positive controlA431, HeLa, or ML-1 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesFrozen Sections Ogawa, H., et al. 2001. Brain Res. 890, 197. Immunoblotting Dallas, P.B., et al. 1997. Hybridoma 16, 273. Immunoprecipitation Abraham, S.E., et al. 1993. Oncogene 8, 1639.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human p300 protein
ImmunogenHuman
Epitopewithin amino acids 2071-2091 near the CBP C-terminus
CloneNM-11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
NA46-100UG 04055977227574

Documentation

Anti-p300 Mouse mAb (NM-11) MSDS

Title

Safety Data Sheet (SDS) 

Anti-p300 Mouse mAb (NM-11) Certificates of Analysis

TitleLot Number
NA46

References

Przegląd literatury
Dallas, P.B., et al. 1997. Hybridoma 16, 273.
<→EMD:if(output-is PDS)→> Shisler, J., et al. 1996. J. Virol. 70, 68.
Wang, H.G., et al. 1995. J. Virol. 69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene 9, 1733.
Eckner, R., et al. 1994. Genes Dev. 8, 869.
Abraham, S.E., et al. 1993. Oncogene 8, 1639.
Wang, H.G., et al. 1993. J. Virol. 67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology 186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol. 11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol. 11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol. 8, 3955.
Yee, S. and Branton, P., 1985. Virology 147, 142. <→EMD:endif()→>
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
ApplicationFrozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.
Backgroundp300 is a nuclear phosphoprotein which was originally shown to specifically bind to adenovirus E1A protein. The binding of p300 to E1A occurs in the conserved region 1 (CR1) of E1A at a site which is close to but non-overlapping with that for the Rb family of proteins including p105 and p107. p300 has been shown to bind to the extreme N-terminal region requiring the presence of positively charged residues at positions 2 and 3 of E1A via a zinc-finger motif in the C-terminal portion of p300. While the binding of p300 and Rb to E1A is independent, it has been shown that upon binding, E1A mediates the physical contact between the two proteins. The p300 protein is actively phosphorylated in both quiescent and proliferating cells however the amount of phosphorylation increases during progression from late G1 into M phase of the cell cycle. In addition to the demonstrated binding of p300 to E1A it has also been shown to bind to other molecules including DNA (in a site specific manner) and to components of the TATA binding protein complexes (TBP). The TBP complex association is most likely with TFIID and occurs in a protein-protein dependent rather than a DNA dependent manner and, in such a complex, p300 functions as a co-activator of transcription.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human p300 protein
Epitopewithin amino acids 2071-2091 near the CBP C-terminus
CloneNM-11
IsotypeIgG₁
Specieshuman, not mouse, rat
Positive controlA431, HeLa, or ML-1 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesDallas, P.B., et al. 1997. Hybridoma 16, 273.
<→EMD:if(output-is PDS)→> Shisler, J., et al. 1996. J. Virol. 70, 68.
Wang, H.G., et al. 1995. J. Virol. 69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene 9, 1733.
Eckner, R., et al. 1994. Genes Dev. 8, 869.
Abraham, S.E., et al. 1993. Oncogene 8, 1639.
Wang, H.G., et al. 1993. J. Virol. 67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology 186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol. 11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol. 11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol. 8, 3955.
Yee, S. and Branton, P., 1985. Virology 147, 142. <→EMD:endif()→>
Application referencesFrozen Sections Ogawa, H., et al. 2001. Brain Res. 890, 197. Immunoblotting Dallas, P.B., et al. 1997. Hybridoma 16, 273. Immunoprecipitation Abraham, S.E., et al. 1993. Oncogene 8, 1639.