Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN540 Anti-mGluR5 Antibody, clone N75/33

Detect mGluR5 using this mouse monoclonal antibody, Anti-mGluR5 Antibody, clone N75/33 validated for use in western blotting & IHC.

Anti-mGluR5 Antibody, clone N75/33 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: Grm5, Gprc1e, Mglur5 UniProt Number: P31424
MABN540
100 µg  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN540
DescriptionAnti-mGluR5 Antibody, clone N75/33
Alternate Names
  • Metabotropic glutamate receptor 5
  • mGluR5
Background InformationMetabotropic glutamate receptor 5 (mGluR5) is a G-protein mediated glutamate receptor that generates a calcium-activated chloride current. mGluR5 is widely distributed in neuronal cells of the central nervous system.
References
Product Information
FormatPurified
Control
  • Hypothalamus tissue lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect mGluR5 using this mouse monoclonal antibody, Anti-mGluR5 Antibody, clone N75/33 validated for use in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected mGluR5 in 10 µg of rat brain membrane tissue.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected mGluR5 in normal human midbrain tissue.
Biological Information
ImmunogenRecombinant protein corresponding to the C-terminus of rat mGluR5.
EpitopeC-terminus
CloneN75/33
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the C-terminus of mGluR5. This antibody may cross react with mGluR1 based upon <35% sequence homology.
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Grm5
  • Gprc1e
  • Mglur5
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~150-270 kDa observed. This protein may be observed at higher molecular weights due to glycosylation (Bhave, G., et al. (2003). J Biol Chem. 278(32):30294-30301). This protein is observed as a dimer at ~270 kDa, but is observed as a monomer at ~140 kDa under reduced conditions in SDS-PAGE (Romano, C., et al. (1996). J Biol Chem. 271(45):28612-28616.). Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse hypothalamus tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected mGluR5 in 10 µg of mouse hypothalamus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABN540 04053252906510

Documentation

Anti-mGluR5 Antibody, clone N75/33 MSDS

Title

Safety Data Sheet (SDS) 

Anti-mGluR5 Antibody, clone N75/33 Certificates of Analysis

TitleLot Number
Anti-mGluR5, clone N75/33 3071910
Anti-mGluR5, clone N75/33 - 3200629 3200629
Anti-mGluR5, clone N75/33 - 3352426 3352426
Anti-mGluR5, clone N75/33 - 3484992 3484992
Anti-mGluR5, clone N75/33 - 3704260 3704260
Anti-mGluR5, clone N75/33 - 3925601 3925601
Anti-mGluR5, clone N75/33 - 4123349 4123349
Anti-mGluR5, clone N75/33 - Q2181577 Q2181577
Anti-mGluR5, clone N75/33 -2584065 2584065
Anti-mGluR5, clone N75/33 -2636737 2636737
Show more

Brochure

Title
Pathways and Biomarkers of Glutamatergic Synapse Flyer