Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

07-1614 Anti-XRCC2 Antibody

Use Anti-XRCC2 Antibody (rabbit polyclonal antibody) validated in IHC(P), WB, ICC to detect XRCC2 also known as DNA repair protein XRCC2, X-ray repair complementing defective repair in Chinese hamster cells 2.

Anti-XRCC2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: DKFZp781P0919 , RAD51-like UniProt Number: O43543
07-1614
100 µL  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIH(P), WB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1614
ReplacesMABE556
DescriptionAnti-XRCC2 Antibody
Alternate Names
  • DNA repair protein XRCC2
  • X-ray repair complementing defective repair in Chinese hamster cells 2
  • X-ray repair cross complementing protein 2
  • X-ray repair cross-complementing protein 2
  • X-ray repair
  • complementing defective
  • repair in Chinese hamster
Background InformationX-ray repair cross-complementing protein (XRCC2) is a member of the RecA family which is essential for DNA repair and maintenance. XRCC2 also belongs to the Rad51 subfamily of proteins involved in double stranded DNA break repair. XRCC2 is thought to encode a member of the RecA/Rad51 related protein family which maintains chromosome stability and repairs DNA damage. XRCC2 and RAD51 interact to form a multimeric ring in the absence of DNA. XRCC2 is thought to load Rad51 onto gapped DNA.
References
Product Information
FormatAffinity Purified
Control
  • Jurkat cell lysate, human lung, NIH/3T3, A431, HeLa cells.
PresentationRabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives
Quality LevelMQ100
Applications
ApplicationUse Anti-XRCC2 Antibody (rabbit polyclonal antibody) validated in IHC(P), WB, ICC to detect XRCC2 also known as DNA repair protein XRCC2, X-ray repair complementing defective repair in Chinese hamster cells 2.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry:
Anti-XRCC2 polyclonal was used in immunocytochemistry to positively stain the nucleus on A431, NIH/3T3 and HeLa cells.

Immunohistochemistry(paraffin): Anti-XRCC2 antibody was used for immunohistochemistry on human lung tissue.
Formalin-fixed and paraffin-embedded Human lung tissue stained with anti-XRCC2 (1:100) with Citrate pH 6.0 (HIER).
Biological Information
ImmunogenA synthetic peptide corresponding to amino acids 206-217 of XRCC2.
HostRabbit
SpecificityThis antibody recognizes XRCC2.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents. [provided by RefSeq].
Gene Symbol
  • DKFZp781P0919
  • RAD51-like
Purification MethodAffinity purified polyclonal precipitated in ammonium sulfate.
UniProt Number
UniProt SummaryFUNCTION: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Ref.6

SUBUNIT STRUCTURE: Interacts with RAD51D. Part of a BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2. Part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3. In the absence of DNA, XRCC2-RAD51D formed a multimeric ring structure. In the presence of single-stranded DNA, XRCC2-RAD51D formed a filamentous structure.

SUBCELLULAR LOCATION: Nucleus.

PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10

SIMILARITY: Belongs to the recA family. RAD51 subfamily.
Molecular Weight~32 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceWestern Blot Analysis:
1:500 dilution of this antibody detected XRCC2 in 10 µg of a Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
07-1614 04053252404740