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GR37 Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)

This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).

Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
GR37
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Przegląd

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
Am, Ch, Ht, H, M, Porcine, RRMonoclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
GR37-100UG Ampulka plastikowa 100 μg
Dodaj do Ulubionych
Description
OverviewRecognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.
Catalogue NumberGR37
Brand Family Calbiochem®
References
ReferencesBrown, A.J. 1999. Nephrol. Dial. Transplant. 14, 11.
Fleet, J.C. 1999. Nutr. Rev. 57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res. 4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem. 46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res. 47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 241.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlBreast carcinoma tissue (see comments)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Gel Shift
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (1:50-1:100)
Gel Shift (see comments)
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Application CommentsDoes not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified, chick intestine vitamin D receptor
ImmunogenChicken
Clone9A7γE10.4
HostRat
IsotypeIgG₁
Species Reactivity
  • Amphibian
  • Chicken
  • Hamster
  • Human
  • Mouse
  • Porcine
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C).
Global Trade Item Number
Numer katalogowy GTIN
GR37-100UG 04055977228311