Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABS82-AF647 Anti-VEGF Antibody, Alexa Fluor® 647 Conjugate

Anti-VEGF Antibody, Alexa Fluor® 647 Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: VEGFA, VEGF UniProt Number: P15692
ABS82-AF647
100 µL  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RICCRbAlexaFluor®647Polyclonal Antibody
Description
Catalogue NumberABS82-AF647
DescriptionAnti-VEGF Antibody, Alexa Fluor® 647 Conjugate
Alternate Names
  • Vascular endothelial growth factor A
  • VEGF-A
  • Vascular permeability factor
  • VPF
  • VEGF, Alexa Fluor® 647 Conjugate
Background InformationVEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively). All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and also plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.
References
Product Information
FormatAlexaFluor®647
PresentationPurified rabbit antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody recognizes VEGF at the N-terminus.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Non-Human Primate based on 100% sequence homology. Other homologies: Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology). Predicted to react with primate based on 100% sequence homology..
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • VEGFA
  • VEGF
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~55 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ABS82-AF647 04055977311945

Documentation

Anti-VEGF Antibody, Alexa Fluor® 647 Conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-VEGF Antibody, Alexa Fluor® 647 Conjugate Certificates of Analysis

TitleLot Number
Anti-VEGF, Alexa Fluor® 647 -Q2636951 Q2636951
Anti-VEGF, Alexa Fluor® 647 Conjugate - 3269420 3269420
Anti-VEGF, Alexa Fluor® 647 Conjugate - 3474109 3474109
Anti-VEGF, Alexa Fluor® 647 Conjugate - 3697934 3697934
Anti-VEGF, Alexa Fluor® 647 Conjugate - 3749943 3749943
Anti-VEGF, Alexa Fluor® 647 Conjugate - 4078187 4078187
Anti-VEGF, Alexa Fluor® 647 Conjugate - 4139146 4139146