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MAB7774 Anti-Urokinase Antibody, NT fragment, clone 10D.7.2

This Anti-Urokinase Antibody, N-terminus fragment, clone 10D.7.2 is validated for use in ELISA, WB for the detection of Urokinase.

Anti-Urokinase Antibody, NT fragment, clone 10D.7.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Enzymes & Biochemistry Gene Symbol: PLAU, UPA, URK, u-PA, uPA, ATF, EC 3.4.21.73 [Contains: Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A, Urokinase-type plasminogen activator chain B]. UniProt Number: P00749
MAB7774
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB7774
ReplacesAB776
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Urokinase Antibody, NT fragment, clone 10D.7.2
Alternate Names
  • uPA
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid in 0.02 M phosphate buffer containing 0.25 M NaCl2 and 0.1% NaN3.
Quality LevelMQ100
Applications
ApplicationThis Anti-Urokinase Antibody, N-terminus fragment, clone 10D.7.2 is validated for use in ELISA, WB for the detection of Urokinase.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1:1000-1:3000

ELISA

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenAmino Terminal Fragment of uPA
EpitopeN-terminus fragment
Clone10D.7.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityUrokinase Plasminogen Acitivator is a serine protease (SERPIN) that converts plasminogen into the active protease plasmin. uPA is secreted from cells in as the inactive 52 kDa single chain precursor which is activated into the two chain form consisting of an alpha and beta chain.MAB7774 is specific for Human uPA, other species not tested.
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer disease and also with decreased affinity for fibrin-binding. The protein encoded by this gene converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. This gene's proprotein is cleaved at a Lys-Ile bond by plasmin to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor.
Gene Symbol
  • PLAU
  • UPA
  • URK
  • u-PA
  • uPA
  • ATF
  • EC 3.4.21.73 [Contains: Urokinase-type plasminogen activator long chain A
  • Urokinase-type plasminogen activator short chain A
  • Urokinase-type plasminogen activator chain B].
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P00749 # Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
SIZE: 431 amino acids; 48525 Da
SUBUNIT: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in the prostate gland and prostate cancers.
PTM: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
SIMILARITY: SwissProt: P00749 ## Belongs to the peptidase S1 family. & Contains 1 EGF-like domain. & Contains 1 kringle domain. & Contains 1 peptidase S1 domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months.
Packaging Information
Material Size100 µg
Global Trade Item Number
Numer katalogowy GTIN
MAB7774 04053252501609