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AB1586 Anti-Ubiquitin Antibody

AB1586
100 µL  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
Eu, H, Rb, ShWB, IHCShSerumPolyclonal Antibody
Description
Catalogue NumberAB1586
Replaces04-454
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Ubiquitin Antibody
References
Product Information
FormatSerum
PresentationSheep serum. Lyophilized, no preservatives. Reconstitute with 100 mL of sterile distilled water. Centrifuge to remove any residue.
Quality LevelMQ100
Applications
ApplicationDetect Ubiquitin using this Anti-Ubiquitin Antibody validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry: 1:2,000-1:5,000.

Immunoblotting: 1:1,000-1:2,000 (depending on detection system used).

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenUbiquitin from bovine red blood cells coupled to chicken gamma globulin.
HostSheep
SpecificityRecognizes both free ubiquitin and ubiquitin-protein conjugates on Western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections.
Species Reactivity
  • Eukaryote
  • Human
  • Rabbit
  • Sheep
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
Gene Symbol
  • UBC
  • UBA80
  • HMG20
  • UBCEP1
  • Ubiquitin.
  • UBA52
  • UBB
  • UBCEP2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
SIZE: 76 amino acids; 8565 Da
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
AB1586 04053252410024

Documentation

Anti-Ubiquitin Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Ubiquitin Antibody Certificates of Analysis

TitleLot Number
AB1586 - 2293057 2293057
SHEEP ANTI-UBIQUITIN POLYCLONAL ANTIBODY - 2106876 2106876
SHEEP ANTI-UBIQUITIN POLYCLONAL ANTIBODY - 2435063 2435063
SHEEP ANTI-UBIQUITIN - 3688554 3688554
SHEEP ANTI-UBIQUITIN - 3853755 3853755
SHEEP ANTI-UBIQUITIN -2776250 2776250
SHEEP ANTI-UBIQUITIN POLYCLONAL ANTIBODY 2999531
SHEEP ANTI-UBIQUITIN POLYCLONAL ANTIBODY - 2193266 2193266
SHEEP ANTI-UBIQUITIN POLYCLONAL ANTIBODY - 2208744 2208744
SHEEP ANTI-UBIQUITIN POLYCLONAL ANTIBODY - 2341056 2341056

References

Reference overviewPub Med ID
WW domain containing E3 ubiquitin protein ligase 1 (WWP1) negatively regulates TLR4-mediated TNF-α and IL-6 production by proteasomal degradation of TNF receptor associated factor 6 (TRAF6).
Lin, XW; Xu, WC; Luo, JG; Guo, XJ; Sun, T; Zhao, XL; Fu, ZJ
PloS one  8  e67633  2013

Pokaż streszczenie
23799152 23799152
The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates.
Haas, A L and Bright, P M
J. Biol. Chem., 260: 12464-73 (1985)  1985

Pokaż streszczenie
2995377 2995377

Data Sheet

Title
SHEEP ANTI-UBIQUITIN POLYCLONAL ANTIBODY