Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABT205 Anti-α-Tubulin Antibody, clone DM1A

This Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin.

Anti-α-Tubulin Antibody, clone DM1A MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton UniProt Number: P02552
MABT205
100 µg  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, ChWB, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT205
Replaces04-1117
DescriptionAnti-α-Tubulin Antibody, clone DM1A
Alternate Names
  • Tubulin alpha-1 chain
Background InformationTubulin is one of the main components of the cytoskeleton. It is a heterodimeric structure consisting of interlocking alpha and beta chains. Like other cytoskeleton proteins, tubulin undergoes dynamic changes depending on the cellular context, which are mediated by binding and release of GDP/GTP. Tubulin proteins may also undergo post-translational modifications, and are essential to cellular transport and mitotic processes.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate.
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected α-tubulin in NIH/3T3, A431, and HeLa cells.

Immunohistochemistry Analysis: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue.
Biological Information
ImmunogenNative chicken brain microtubules.
CloneDM1A
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Chicken
Species Reactivity NoteDemonstrated to react wtih Human and Mouse. Predicted to react with Chicken based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

SUBUNIT STRUCTURE: Dimer of alpha and beta chains.

SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton.

POST-TRANSLATIONAL MODIFICATION: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.

MISCELLANEOUS: There are at least seven alpha tubulin genes (alpha-1 to alpha-6, and alpha-8), and a pseudogene (alpha-7) in chicken.

SEQUENCE SIMILARITIES: Belongs to the tubulin family.
Molecular Weight~60 kDa observed. Uniprot describes a molecular weight at ~46 kDA, but can be higher due to post-translational modification. This protein has been observed at ~55 kDa in some cell lysates (Saugstad, J. A., et al. (2002). J Neurochem. 80(6):980-988.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABT205 04053252549328

Documentation

Anti-α-Tubulin Antibody, clone DM1A MSDS

Title

Safety Data Sheet (SDS) 

Anti-α-Tubulin Antibody, clone DM1A Certificates of Analysis

TitleLot Number
Anti--Tubulin, clone DM1A - 2204989 2204989
Anti--Tubulin, clone DM1A - 2294350 2294350
Anti--Tubulin, clone DM1A - Q1999234 Q1999234
Anti-α-Tubulin, clone DM1A - 2424847 2424847
Anti-α-Tubulin, clone DM1A - 2494100 2494100
Anti-α-Tubulin, clone DM1A - 3173554 3173554
Anti-α-Tubulin, clone DM1A - 3286042 3286042
Anti-α-Tubulin, clone DM1A - 3482839 3482839
Anti-α-Tubulin, clone DM1A - 3671775 3671775
Anti-α-Tubulin, clone DM1A - 3777313 3777313
Show more

Brochure

Title
New Products: Volume 3, 2012