Ilość	Numer katalogowy	Opis zamawiania	Ilość/opak.	Lista cen
			96-Well Plate

Ilość	Numer katalogowy	Opis zamawiania	Ilość/opak.	Lista cen
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

09-126 Sigma-AldrichAnti-Tribbles-1 Antibody

Anti-Tribbles-1, Cat. No. 09-126, is a rabbit polyclonal antibody that detects Tribbles-1 and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.

Anti-Tribbles-1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: TRIB1, TRB1, SKIP1, C8FW, GIG2 UniProt Number: Q96RU8

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Tabela kluczowych gatunków

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, PIA, IH(P)	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	09-126
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Tribbles-1 Antibody
Background Information	Tribbles homolog 1 (UniProt: Q96RU8; also known as TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1) is encoded by the TRIB1 (also known as C8FW, GIG2, TRB1) gene (Gene ID: 10221) in human. TRB-1 is an evolutionary conserved monomeric protein that acts as a pseudokinase. It is widely distributed, but highest concentrations are found in the bone marrow and the thyroid gland. In mammals, it controls several interacting signaling pathways, including MAP kinases and PI-3 kinase pathways. In the N-terminal region it contains a PEST region and a pseudokinase domain. In the C-terminal region it has a COP1-binding region (aa 355-360). Due to the lack of a functional ATP-binding site it is catalytically inactive but serves as an adapter or scaffold that facilitates assembly of other proteins. Its COP1-binding motif is masked by autoinhibitory interactions with the protein kinase domain. TRB-1 has been established as an oncogene in acute myeloid leukemia and his reported to play a pivotal role in the differentiation of anti-inflammatory M2-macrophages in the pathogenesis of early atherosclerosis and during adipose tissue inflammation. It is also shown to be overexpressed in prostate cancer and is associated with cancer risk. (Ref.: Niespolo, C.., et a. (2020). Front. Immunol. 11; 574046; Kiss-Toth, E., et al. (2004). J. Biol. Chem. 279(41); 42703-42708).

References

Product Information
Format	Affinity Purified
Control	HeLa
Presentation	Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Tribbles-1, Cat. No. 09-126, is a rabbit polyclonal antibody that detects Tribbles-1 and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
Key Applications	Western Blotting Peptide Inhibition Assay Immunohistochemistry (Paraffin)
Application Notes	Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Tribbles-1 in Human Skeletal Muscle tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:250 Dilution from a representative lot detected Tribbles-1 in human atherosclerotic aorta tissue sections. Peptide Inhibition Assay: Target band detection in lysate of Human skeletal muscle tissue was prevented by preblocking of a representative lot with the immunogen peptide. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Tribbles homolog 1 (TRB-1).
Epitope	N-terminal
Host	Rabbit
Specificity	This rabbit polyclonal antibody specifically detects human Tribbles-1 (TRB-1). It targets an epitope within 14 amino acids from the N-terminal region.
Isotype	IgG
Species Reactivity	Human
Species Reactivity Note	Human.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_079471
Gene Symbol	TRIB1 TRB1 SKIP1 C8FW GIG2
Purification Method	Affinity Purfied
UniProt Number	Q96RU8
Molecular Weight	Target molecular weight ~47 kDa observed. 41.01 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in acid extract of HeLa cells. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tribbles-1 in Acid extract of HeLa cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Numer katalogowy	GTIN
09-126	04053252669750

Documentation

Anti-Tribbles-1 Antibody MSDS

Title
Safety Data Sheet (SDS)

Anti-Tribbles-1 Antibody Certificates of Analysis

Title	Lot Number
Anti-Tribbles-1 - 2142242	2142242
Anti-Tribbles-1 - 2041023	2041023
Anti-Tribbles-1 - 2193205	2193205
Anti-Tribbles-1 - 2266822	2266822
Anti-Tribbles-1 - 2332544	2332544
Anti-Tribbles-1 - 3311804	3311804
Anti-Tribbles-1 - 3698134	3698134
Anti-Tribbles-1 - 3834630	3834630
Anti-Tribbles-1 - DAM1469775	DAM1469775
Anti-Tribbles-1 - DAM1614955	DAM1614955

References

Reference overview	Application	Pub Med ID
Enhanced macrophage tribbles-1 expression in murine experimental atherosclerosis. Sung, HY; Francis, SE; Arnold, ND; Holland, K; Ernst, V; Angyal, A; Kiss-Toth, E Biology 1 43-57 2011 Pokaż streszczenie Development of the atherosclerotic plaque involves a complex interplay between a number of cell types and an extensive inter-cellular communication via cell bound as well as soluble mediators. The family of tribbles proteins has recently been identified as novel controllers of pro-inflammatory signal transduction. The objective of this study was to address the expression pattern of all three tribbles proteins in atherosclerotic plaques from a mouse model of atherosclerosis. Each tribbles were expressed in vascular smooth muscle cells, endothelial cells as well as in resident macrophages of mouse atherosclerotic plaques. The role of IL-1 mediated inflammatory events in controlling tribbles expression was also addressed by inducing experimental atherosclerosis in ApoE-/-IL1R1-/- (double knockout) mice. Immunohistochemical analysis of these mice showed a selective decrease in the percentage of trb-1 expressing macrophages, compared to the ApoE-/- cohort (14.7% ± 1.55 vs. 26.3% ± 1.19). The biological significance of this finding was verified in vitro where overexpression of trb-1 in macrophages led to a significant attenuation (~70%) of IL-6 production as well as a suppressed IL-12 expression induced by a proinflammatory stimulus. In this in vitro setting, expression of truncated trb-1 mutants suggests that the kinase domain of this protein is sufficient to exert this inhibitory action.	Western Blotting	24832046

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