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612161 Anti-Tissue Factor Mouse mAb (TF9-10H10)

This Anti-Tissue Factor Mouse mAb (TF9-10H10) is validated for use in Dot Blot, ELISA, FC, Frozen Sections, WB, IF, Paraffin Sections, Neutralization Studies for the detection of Tissue Factor.

Anti-Tissue Factor Mouse mAb (TF9-10H10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
612161
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Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
H, PmMMonoclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
612161-500UG Ampulka plastikowa 500 μg
Description
OverviewRecognizes the tissue factor protein.
Catalogue Number612161
Brand Family Calbiochem®
Application Data
Detection of human tissue factor by immunoblotting. Sample: Whole cell lysate from JEG-3 cells. Primary antibody: Anti-Tissue Factor Mouse mAb (TF9-10H10) (Cat. No. 612161) (1:1000). Detection: chemiluminescence.
References
Product Information
FormLyophilized
FormulationLyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesFlow Cytometry Veltrop, M.H., et al. 2001. Infect. Immun. 69, 2797. ELISA, Frozen Sections, Paraffin Sections, Immunocytochemistry Contrino, J., et al. 1994. Am. J. Pathol. 145, 1315. Original Clone, Immunoblotting, Dot Blot, Inhibition Studies, Neutralization Studies Morrissey, J.H., et al. 1988. Thromb. Res. 52, 247. Immunoblotting Ott, I., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 20, 874.
Key Applications Dot Blot
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Not Neutralization Studies
Paraffin Sections
Application NotesDot Blot (see application references)
ELISA (see application references)
Flow Cytometry (see application references)
Frozen Sections (see application references)
Immunoblotting (1:1000, see application references)
Immunofluorescence (see application references)
Paraffin Sections (see application references)
Neutralization Studies (not recommended)
Application CommentsSpecific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified apoprotein of human tissue factor
ImmunogenHuman
Epitopewithin extracellular domain
CloneTF9-10H10
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Primate
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
612161-500UG 04055977187748

Documentation

Anti-Tissue Factor Mouse mAb (TF9-10H10) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Tissue Factor Mouse mAb (TF9-10H10) Certificates of Analysis

TitleLot Number
612161
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-January-2016 JSW
ApplicationDot Blot (see application references)
ELISA (see application references)
Flow Cytometry (see application references)
Frozen Sections (see application references)
Immunoblotting (1:1000, see application references)
Immunofluorescence (see application references)
Paraffin Sections (see application references)
Neutralization Studies (not recommended)
Application Data
Detection of human tissue factor by immunoblotting. Sample: Whole cell lysate from JEG-3 cells. Primary antibody: Anti-Tissue Factor Mouse mAb (TF9-10H10) (Cat. No. 612161) (1:1000). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the tissue factor protein.
BackgroundTissue factor (TF) is the membrane receptor for coagulation factors VII and VIIa and is a cell-surface initiator of coagulation. It is the major molecule of this type and is critical for controlling hemostasis, thrombosis, and inflammation. TF is not generally present in or on cells within the vasculature, but following vascular injury, blood can come into contact with TF expressed by extravascular cells, resulting in activation of the coagulation cascade.
HostMouse
Immunogen speciesHuman
Immunogenpurified apoprotein of human tissue factor
Epitopewithin extracellular domain
CloneTF9-10H10
IsotypeIgG₁
Specieshuman, primate, not rabbit
FormLyophilized
FormulationLyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.
PreservativeNone
CommentsSpecific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
Application referencesFlow Cytometry Veltrop, M.H., et al. 2001. Infect. Immun. 69, 2797. ELISA, Frozen Sections, Paraffin Sections, Immunocytochemistry Contrino, J., et al. 1994. Am. J. Pathol. 145, 1315. Original Clone, Immunoblotting, Dot Blot, Inhibition Studies, Neutralization Studies Morrissey, J.H., et al. 1988. Thromb. Res. 52, 247. Immunoblotting Ott, I., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 20, 874.