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MAB19101 Anti-Tenascin Antibody, clone DB7

MAB19101
50 µg  
Purchase on Sigma-Aldrich

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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
Gp, H, RbIF, WB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB19101
ReplacesMAB1902
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tenascin Antibody, clone DB7
Alternate Names
  • Tenascin-C
Product Information
FormatPurified
PresentationThe purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P).
Key Applications
  • Immunofluorescence
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.

Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.

Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.

Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)

Final dilutions must be determined by end user.
Biological Information
ImmunogenTenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography.
CloneDB7
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThe antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein
IsotypeIgG2a
Species Reactivity
  • Guinea Pig
  • Human
  • Rabbit
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ITGAM
  • MO1A
  • CD11b
  • CR3A
  • MAC1A
  • CD11B
  • MGC117044
  • MAC-1
Non-Reactive Species
  • Mouse
  • Rat
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P11215 # Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
SIZE: 1152 amino acids; 127179 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Predominantly expressed in monocytes and granulocytes.
DOMAIN: SwissProt: P11215 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C
Packaging Information
Material Size50 µg
Global Trade Item Number
Numer katalogowy GTIN
MAB19101 04053252577659