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AB5454 Anti-TUC-4 Protein Antibody

AB5454
100 µL  
Purchase on Sigma-Aldrich

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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Mk, R, FeICC, IHC, IP, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberAB5454
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-TUC-4 Protein Antibody
Alternate Names
  • ULIP-1 Protein
  • CRMP-4
  • DRP-3
  • Dihydropyrimidinase-like 3
Background InformationTUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (Quinn, 1999). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (Minturn, 1995a). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (Minturn, 1995b). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (Quinn, 1999). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (Minturn, 1995a). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (Minturn, 1995b) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR; Gaetano, 1997).
References
Product Information
FormatSerum
Control
  • POSITIVE CONTROL: Fetal brain/spinal cord tissue. Human neuroblastoma cell line SMS-KCNR or rat PC12 cells.
PresentationRabbit Serum. Liquid containing 0.2% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TUC-4 Protein Antibody detects level of TUC-4 Protein & has been published & validated for use in IC, IH, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry: 1:1,000-1:5,000 using PFA (DAB detection).
Immunocytochemistry: 1:1,000-1:5,000 (DAB detection).
Western blot: 1:2,500-1:25,000 using alkaline phosphatase.
Immunoprecipitation

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK)
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificitySpecifically recognizes the TUC-4 (Ulip-1/CRMP-4/DRP-3) splice variants, TUC-4a (64 kDa) and the N-terminally extended TUC-4b (75 kDa) (Quinn, 2003). AB5454 has been reported to label early postmitotic neurons in prenatal rat brain. Labeling is observed primarily within the cytoplasm along the most distal regions of neuronal growth cones including lamellipodia and filopodia. The antibody is not reactive with the proteins TUC-1 (Ulip-3/CRMP-1), TUC-2 (Ulip-2/CRMP-2) or TUC-3 (Ulip-4/CRMP-3). It does not label neural progenitors or radial glia.
Species Reactivity
  • Human
  • Mouse
  • Monkey
  • Rat
  • Feline
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DPYSL3
  • CRMP-4
  • DRP3
  • CRMP4
  • ULIP
  • DRP-3
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q14195 # May regulate vesicle function in the growth cone (By similarity).
SIZE: 570 amino acids; 61963 Da
SUBUNIT: Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, growth cone (By similarity). Note=Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone (By similarity).
TISSUE SPECIFICITY: Mainly in heart and skeletal muscle. Also expressed strongly in fetal brain and spinal cord.
SIMILARITY: SwissProt: Q14195 ## Belongs to the dehydropyrimidinase family.
Molecular Weight64 & 74 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
AB5454 04053252368431

Documentation

Anti-TUC-4 Protein Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-TUC-4 Protein Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) - 3257099 3257099
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) - 3691496 3691496
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) - 3891787 3891787
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) - 4133130 4133130
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) -2798363 2798363
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) -2809951 2809951
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) POLYCLONAL ANTIBODY 3096496
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) POLYCLONAL ANTIBODY 2941114
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) POLYCLONAL ANTIBODY 2896743
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4) POLYCLONAL ANTIBODY - 2436338 2436338

References

Reference overviewApplicationSpeciesPub Med ID
Crmp4 deletion promotes recovery from spinal cord injury by neuroprotection and limited scar formation.
Nagai, J; Kitamura, Y; Owada, K; Yamashita, N; Takei, K; Goshima, Y; Ohshima, T
Scientific reports  5  8269  2015

Pokaż streszczenie
Immunofluorescence25652774 25652774
Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.
Laskowska-Macios, K; Nys, J; Hu, TT; Zapasnik, M; Van der Perren, A; Kossut, M; Burnat, K; Arckens, L
Molecular brain  8  48  2015

Pokaż streszczenie
26271461 26271461
Recovery of brain biomarkers following peroxisome proliferator-activated receptor agonist neuroprotective treatment before ischemic stroke.
Gelé, P; Vingtdeux, V; Potey, C; Drobecq, H; Ghestem, A; Melnyk, P; Buée, L; Sergeant, N; Bordet, R
Proteome science  12  24  2014

Pokaż streszczenie
24944524 24944524
Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1.
Gokhale, A; Larimore, J; Werner, E; So, L; Moreno-De-Luca, A; Lese-Martin, C; Lupashin, VV; Smith, Y; Faundez, V
The Journal of neuroscience : the official journal of the Society for Neuroscience  32  3697-711  2011

Pokaż streszczenie
Immunofluorescence22423091 22423091
Comparative proteomics of spinal cords of rat fetuses with spina bifida aperta.
Yang Fan,Lili Wang,Fenghua Zhou,Yi Zhang,Hui Li,Liping Shan,Zhengwei Yuan
Journal of proteomics  75  2010

Pokaż streszczenie
21945729 21945729
Heterogeneous intrastriatal pattern of proteins regulating axon growth in normal adult human brain.
Tong, J; Furukawa, Y; Sherwin, A; Hornykiewicz, O; Kish, SJ
Neurobiology of disease  41  458-68  2010

Pokaż streszczenie
21029775 21029775
Expression of neurogenesis genes in human temporal lobe epilepsy with hippocampal sclerosis.
Engel T, Schindler CK, Sanz-Rodriguez A, Conroy RM, Meller R, Simon RP, Henshall DC
Int J Physiol Pathophysiol Pharmacol  3  38-47. Epub 2010 Dec 15  2010

Pokaż streszczenie
21479101 21479101
Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy.
Moreira JM, Cabezón T, Gromova I, Gromov P, Timmermans-Wielenga V, Machado I, Llombart-Bosch A, Kroman N, Rank F, Celis JE
Mol Oncol  4  539-61. Epub 2010 Oct 8.  2009

Pokaż streszczenie
21036680 21036680
Circumventricular organs: a novel site of neural stem cells in the adult brain.
Bennett, L; Yang, M; Enikolopov, G; Iacovitti, L
Molecular and cellular neurosciences  41  337-47  2009

Pokaż streszczenie
19409493 19409493
Genesis of neuronal and glial progenitors in the cerebellar cortex of peripuberal and adult rabbits.
Ponti, G; Peretto, P; Bonfanti, L
PloS one  3  e2366  2008

Pokaż streszczenie
Rabbit18523645 18523645

Data Sheet

Title
RABBIT ANTI-TUC-4 PROTEIN (Ulip-1/CRMP-4)